Institute of Aging Research, Guangdong Medical College, Dongguan, P,R, China.
Lipids Health Dis. 2014 Jun 7;13:94. doi: 10.1186/1476-511X-13-94.
Abnormal lipids is one of the critical risk factors for myocardial infarction (MI), however the role of genetic variants in lipid metabolism-related genes on MI pathogenesis still requires further investigation. We herein genotyped three SNPs (LRP6 rs2302685, LDLRAP1 rs6687605, SOAT1 rs13306731) in lipid metabolism-related genes, aimed to shed light on the influence of these SNPs on individual susceptibility to MI.
Genotyping of the three SNPs (rs2302685, rs6687605 and rs13306731) was performed in 285 MI cases and 650 control subjects using polymerase chain reaction-ligation detection reaction (PCR-LDR) method. The association of these SNPs with MI and lipid profiles was performed with SPSS software.
Multivariate logistic regression analysis showed that C allele (OR = 1.62, P = 0.039) and the combined CT/CC genotype (OR = 1.67, P = 0.035) of LRP6 rs2302685 were associated with increased MI risk, while the other two SNPs had no significant effect. Further stratified analysis uncovered a more evident association with MI risk among younger subjects (≤60 years old). Fascinatingly, CT/CC genotype of rs2302685 conferred increased LDL-C levels compared to TT genotype (3.0 mmol/L vs 2.72 mmol/L) in younger subjects.
Our data provides the first evidence that LRP6 rs2302685 polymorphism is associated with an increased risk of MI in Chinese subjects, and the association is more evident among younger individuals, which probably due to the elevated LDL-C levels.
异常的血脂是心肌梗死（MI）的关键危险因素之一，然而，脂质代谢相关基因中的遗传变异在 MI 发病机制中的作用仍需要进一步研究。我们在此对脂质代谢相关基因中的三个 SNP（LRP6 rs2302685、LDLRAP1 rs6687605、SOAT1 rs13306731）进行了基因分型，旨在阐明这些 SNP 对个体 MI 易感性的影响。
采用聚合酶链反应-连接酶检测反应（PCR-LDR）法对 285 例 MI 病例和 650 例对照进行了三个 SNP（rs2302685、rs6687605 和 rs13306731）的基因分型。采用 SPSS 软件分析这些 SNP 与 MI 和血脂谱的关系。
多因素 logistic 回归分析显示，LRP6 rs2302685 的 C 等位基因（OR=1.62，P=0.039）和 CT/CC 基因型（OR=1.67，P=0.035）与 MI 风险增加相关，而其他两个 SNP 则没有显著影响。进一步的分层分析显示，在年轻人群（≤60 岁）中，与 MI 风险的相关性更为明显。有趣的是，与 TT 基因型相比，rs2302685 的 CT/CC 基因型在年轻人群中使 LDL-C 水平升高（3.0 mmol/L 比 2.72 mmol/L）。
我们的数据首次提供了证据，表明 LRP6 rs2302685 多态性与中国人群 MI 风险增加相关，并且在年轻人群中更为明显，这可能是由于 LDL-C 水平升高所致。
