School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
Carbohydr Polym. 2014 Sep 22;110:231-7. doi: 10.1016/j.carbpol.2014.03.081. Epub 2014 Apr 2.
Two new Bi3+-Hericium erinaceus polysaccharide (BiHEP) complexes were prepared using Bi3+ and two purified polysaccharides from H. erinaceus (HEPs), respectively. The complexes were characterized by elemental analysis, FT-IR, CD, SEM, AFM, XRD, and TG. The anti-Helicobacter pylori (Hp) activities in vitro by agar dilution assay of the complexes were evaluated. The molecular weights of HEPs were 197 and 20 kDa, respectively. All the analyses confirmed the formation of new BiHEP complexes with lower content of Bi3+ compared with colloidal bismuth subcitrate (CBS), the most utilized bismuth preparation clinically. Furthermore, HEPs themselves have definite inhibition effects on Hp, and BiHEP complexes have lower content of Bi exhibited strong inhibition effects on Hp (MIC=20 μg/mL), similar to that of CBS with higher content of Bi. The study provides a basis for further development of multiple treatments of Hp infection or new medicines.
两种新的 Bi3+- 猴头菇多糖（BiHEP）复合物分别使用 Bi3+ 和从猴头菇中提取的两种纯化多糖（HEPs）制备。通过元素分析、FT-IR、CD、SEM、AFM、XRD 和 TG 对复合物进行了表征。通过琼脂稀释法评估了复合物的体外抗幽门螺杆菌（Hp）活性。HEPs 的分子量分别为 197 和 20 kDa。所有分析均证实形成了新的 BiHEP 复合物，与临床最常用的铋制剂胶体次枸橼酸铋（CBS）相比，Bi3+ 的含量较低。此外，HEPs 本身对 Hp 具有一定的抑制作用，而 BiHEP 复合物的 Bi 含量较低，对 Hp 具有较强的抑制作用（MIC=20 μg/mL），与 Bi 含量较高的 CBS 相似。该研究为进一步开发 Hp 感染的多种治疗方法或新药提供了依据。
