Department of Sanitary and Environmental Engineering, Universidade Federal de Minas Gerais (UFMG), Av. Antonio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Bioresour Technol. 2014 Aug;166:103-11. doi: 10.1016/j.biortech.2014.05.043. Epub 2014 May 21.
The effects of phenol on the nitrogen removal performance of a sequencing batch reactor (SBR) with anammox activity and on the microbial community within the reactor were evaluated. A phenol concentration of 300 mg L(-1) reduced the ammonium-nitrogen removal efficiency of the SBR from 96.5% to 47%. The addition of phenol changed the microbial community structure and composition considerably, as shown by denaturing gradient gel electrophoresis and 454 pyrosequencing of 16S rRNA genes. Some phyla, such as Proteobacteria, Verrucomicrobia, and Firmicutes, increased in abundance, whereas others, such as Acidobacteria, Chloroflexi, Planctomycetes, GN04, WS3, and NKB19, decreased. The diversity of the anammox bacteria was also affected by phenol: sequences related to Candidatus Brocadia fulgida were no longer detected, whereas sequences related to Ca. Brocadia sp. 40 and Ca. Jettenia asiatica persisted. These results indicate that phenol adversely affects anammox metabolism and changes the bacterial community within the anammox reactor.
评估了苯酚对具有厌氧氨氧化活性的序批式反应器（SBR）的脱氮性能以及反应器内微生物群落的影响。苯酚浓度为 300mg/L 时，SBR 的氨氮去除效率从 96.5%降低到 47%。苯酚的添加极大地改变了微生物群落结构和组成，这可以通过变性梯度凝胶电泳和 16S rRNA 基因的 454 焦磷酸测序来证明。一些门，如变形菌门、疣微菌门和厚壁菌门，丰度增加，而其他门，如酸杆菌门、绿弯菌门、浮霉菌门、GN04、WS3 和 NKB19，丰度降低。苯酚还影响厌氧氨氧化菌的多样性：不再检测到与 Candidatus Brocadia fulgida 相关的序列，而与 Ca. Brocadia sp. 40 和 Ca. Jettenia asiatica 相关的序列仍然存在。这些结果表明，苯酚对厌氧氨氧化代谢有不利影响，并改变了厌氧氨氧化反应器内的细菌群落。
