State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
mSystems. 2024 Sep 17;9(9):e0099924. doi: 10.1128/msystems.00999-24. Epub 2024 Aug 27.
, a globally distributed bacterial phylum-level lineage, is poorly understood. Here, we established a comprehensive genomic catalog of , including a total of seven clades (or families) with 179 genomes, and explored the metabolic potential and evolutionary history of these organisms. We show that a single genome, especially those belonging to Clade 6, often encodes multiple hydrogenases with genomes in Clade 2, which rarely encode hydrogenases being the exception. Notably, most members of contain a group A3 [FeFe]-hydrogenase (BfuABC) with a non-canonical electron bifurcation mechanism, in addition to substrate-level phosphorylation and electron transport-linked phosphorylation pathways, in energy conservation. Furthermore, we show that BfuABC from fall into five sub-types. Phylogenetic analysis reveals five independent routes for the evolution of BfuABC homologs in . We speculate that the five sub-types of BfuABC might be acquired from (synonym ) through separate horizontal gene transfer events. These data shed light on the diversity and evolution of bifurcating [FeFe]-hydrogenases and provide insight into the strategy of to adapt to survival in various habitats.
The phylum is widely distributed in various environments. However, their physiology, ecology, and evolutionary history remain unknown, primarily due to the limited availability of the genomes and the lack of cultured representatives of the phylum. Our results have increased the knowledge of the genetic and metabolic diversity of these organisms and shed light on their diverse energy conservation strategies, especially those involving electron bifurcation with a non-canonical mechanism, which are likely responsible for their wide distribution. Besides, the organization and phylogenetic relationships of gene clusters coding for BfuABC in provide valuable clues to the evolutionary history of group A3 electron bifurcating [FeFe]-hydrogenases.
，一个在全球范围内分布的细菌门水平谱系，了解甚少。在这里，我们建立了一个全面的 基因组目录，包括七个进化枝（或科），共 179 个基因组，并探索了这些生物体的代谢潜力和进化历史。我们表明，单个基因组，特别是那些属于进化枝 6 的基因组，通常编码多个氢化酶，而属于进化枝 2 的基因组很少编码氢化酶是例外。值得注意的是， 中的大多数成员包含一个 A3 [FeFe]-氢化酶（BfuABC），具有非典型的电子分叉机制，除了底物水平磷酸化和电子传递连接的磷酸化途径，在能量守恒中。此外，我们表明， 中的 BfuABC 分为五个亚类。系统发育分析揭示了 在进化过程中 BfuABC 同源物的五条独立途径。我们推测，BfuABC 的五个亚型可能是通过独立的水平基因转移事件从 （同义词 ）获得的。这些数据揭示了分叉 [FeFe]-氢化酶的多样性和进化，并为了解 适应各种生境的生存策略提供了线索。
门广泛分布于各种环境中。然而，它们的生理学、生态学和进化历史仍然未知，主要是因为基因组的可用性有限，而且该门的培养代表物缺乏。我们的研究结果增加了对这些生物体遗传和代谢多样性的了解，并揭示了它们多样化的能量守恒策略，特别是涉及具有非典型机制的电子分叉的策略，这可能是它们广泛分布的原因。此外， 中编码 BfuABC 的基因簇的组织和系统发育关系为 A3 电子分叉 [FeFe]-氢化酶的进化历史提供了有价值的线索。
