Buckley E, Reilly P, Rodriguez Flores J, O'Kennedy R, Smyth M R
School of Chemical Sciences, NIHE Dublin, Glasnevin, Ireland.
J Pharm Biomed Anal. 1989;7(5):543-7. doi: 10.1016/0731-7085(89)80219-5.
The adsorptive stripping voltammetric behaviour of F(ab')2 and Fab fragments of anti-mouse immunoglobulin G is described. Conditions were optimized for the determination of these fragments with respect to accumulation potential, accumulation time, scan rate, pulse amplitude, drop size and electrolyte composition. The F(ab')2 and Fab fragments gave rise to behaviour similar to that reported previously for the intact immunoglobulin.
描述了抗小鼠免疫球蛋白G的F(ab')2和Fab片段的吸附溶出伏安行为。针对这些片段的测定,在富集电位、富集时间、扫描速率、脉冲幅度、液滴大小和电解质组成方面优化了条件。F(ab')2和Fab片段产生的行为与先前报道的完整免疫球蛋白的行为相似。