Nasu H, Chia D S, Knutson D W, Barnett E V
Clin Exp Immunol. 1980 Nov;42(2):378-86.
Antibodies to the F(ab')2 portion of IgG were detected in the sera of patients and normals using a heat-aggregated F(ab')2 fragment in a liquid-phase radioimmunoassay. The antibodies were found in 72% of 82 rheumatoid arthritis patients, in less than 20% of patients with other diseases and in a few normal subjects. Anti-F(ab')2 antibodies were distinct from previously described anti-Fc antibodies and 'serum agglutinators'. They reacted with intact IgG, F(ab')2 and IgM in binding and inhibition assays and they appeared to have specificity for the Fd portion of IgG molecules, possibly to the VH region. The anti-F(ab')2 antibodies were of both 7S IgG and 19S IgM classes and were found in sera as components of intermediate size (between 7S and 19S) and large size (greater than 19S) immune complexes. The possible contribution of autologous anti-F(ab')2 to abnormal immunoregulation is discussed.
在液相放射免疫测定中，使用热聚集的F(ab')2片段检测患者和正常人血清中针对IgG的F(ab')2部分的抗体。在82例类风湿性关节炎患者中，72%的患者血清中发现了这些抗体，其他疾病患者中该比例不到20%，少数正常受试者血清中也有发现。抗F(ab')2抗体与先前描述的抗Fc抗体和“血清凝集素”不同。在结合和抑制试验中，它们能与完整的IgG、F(ab')2和IgM发生反应，并且似乎对IgG分子的Fd部分具有特异性，可能是对VH区域具有特异性。抗F(ab')2抗体属于7S IgG和19S IgM两类，在血清中以中等大小（7S至19S之间）和大尺寸（大于19S）免疫复合物的成分形式存在。本文讨论了自体抗F(ab')2对异常免疫调节的可能作用。
