School of Materials Science and Engineering, Shandong University of Technology, 255049 Shandong, PR China.
School of Science, Shandong University of Technology, 255049 Shandong, PR China.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:283-91. doi: 10.1016/j.msec.2014.04.057. Epub 2014 May 6.
The formations of non-metabolic stones, bones and teeth were seriously related to the morphology, size and surface reactivity of dicalcium phosphate dihydrate (DCPD). Herein, a facile biomimetic mineralization method with presence of glutamic acid and arginine was employed to fabricate DCPD with well-defined morphology and adjustable crystallite size. In reaction solution containing more arginine, crystallization of DCPD occurred with faster rate of nucleation and higher density of stacked layers due to the generation of more OH(-) ions after hydrolysis of arginine at 37 °C. With addition of fluorescein or acetone, the consumption of OH(-) ions or desolvation reaction of Ca(2+) ions was modulated, which resulted in the fabrication of DCPD with adjustable crystallite sizes and densities of stacked layers. In comparison with fluorescein-loading DCPD, dicalcium phosphate anhydrate was prepared with enhanced photoluminescence properties due to the reduction of self-quenching effect and regular arrangement of encapsulated fluorescein molecules. With addition of more acetone, DCPD was prepared with smaller crystallite size via antisolvent crystallization. The simulated process with addition of amino acids under 37 °C would shed light on the dynamic process of biomineralization for calcium phosphate compounds.
二水磷酸氢钙（DCPD）的形态、尺寸和表面反应性与非代谢性结石、骨骼和牙齿的形成密切相关。在此，我们采用了一种简单的仿生矿化方法，在谷氨酸和精氨酸的存在下，制备了具有明确形貌和可调结晶度的 DCPD。在含有更多精氨酸的反应溶液中，由于精氨酸在 37°C 下水解后产生更多的 OH(-)离子，DCPD 的结晶具有更快的成核速率和更高的堆叠层密度。通过添加荧光素或丙酮，可以调节 OH(-)离子的消耗或 Ca(2+)离子的去溶剂化反应，从而制备出具有可调结晶度和堆叠层密度的 DCPD。与负载荧光素的 DCPD 相比，由于自猝灭效应的降低和封装的荧光素分子的规则排列，无水磷酸氢钙具有增强的光致发光性能。通过添加更多的丙酮，通过反溶剂结晶制备出具有较小结晶度的 DCPD。在 37°C 下添加氨基酸的模拟过程将为磷酸钙化合物的生物矿化动态过程提供启示。
