Takabait Fatah, Martínez-Martínez Sergio, Mahtout Laila, Graba Zahra, Sánchez-Soto Pedro J, Pérez-Villarejo Luis
Laboratoire de Technologie des Matériaux et de Génie des Procédés (LTMGP), Faculté des Sciences Exactes, Université A. Mira-Béjaïa, Terga Ouzemmour, Béjaïa 06000, Algeria.
Department of Chemical, Environmental and Materials Engineering, Higher Polytechnic School of Linares, University of Jaén, Campus Científico y Tecnológico, Cinturón Sur s/n, 23700 Linares, Spain.
Materials (Basel). 2023 Feb 1;16(3):1262. doi: 10.3390/ma16031262.
Calcium phosphate (CaP) with several chemical compositions and morphologies was prepared by precipitation using aqueous solutions of L-Glutamic acid (HG) and calcium hydroxide, both mixed together with an aqueous solution (0.15 M) of phosphoric acid. Plate-shaped dicalcium phosphate dihydrate (brushite) particles were obtained and identified at a lower concentration of the solution of the reactants. The Ca/P ratio deduced by EDS was ~1, as expected. The nanoscale dimension of carbonate apatite and amorphous calcium phosphate, with variable Ca/P ratios, were revealed by X-ray diffraction (XRD) and scanning electron microscopy and energy dispersive X-ray spectroscopy analysis (SEM-EDS). They were characterized in medium and high concentrations of calcium hydroxide (0.15 M and 0.20 M). The equilibria involved in all the reactions in aqueous solution were determined. The thermodynamic calculations showed a decrease in the amount of chelate complexes with an increase in pH, being the opposite of [CaPO] and [CaHG]. This fluctuation showed an evident influence on the morphology and polymorphism of CaP particles obtained under the present experimental conditions, with potential use as a biomaterial.
通过使用L-谷氨酸（HG）水溶液和氢氧化钙，二者与磷酸水溶液（0.15 M）混合，经沉淀法制备了具有多种化学成分和形态的磷酸钙（CaP）。在反应物溶液浓度较低时获得并鉴定出了板状二水磷酸二钙（透钙磷石）颗粒。如预期的那样，通过能谱仪（EDS）推导得出的钙磷比约为1。通过X射线衍射（XRD）、扫描电子显微镜和能量色散X射线光谱分析（SEM-EDS）揭示了具有可变钙磷比的碳酸盐磷灰石和无定形磷酸钙的纳米级尺寸。它们在中等和高浓度氢氧化钙（0.15 M和0.20 M）条件下得到了表征。确定了水溶液中所有反应所涉及的平衡。热力学计算表明，随着pH值升高，螯合物络合物的量减少，这与[CaPO]和[CaHG]的情况相反。这种波动对在当前实验条件下获得的CaP颗粒的形态和多晶型具有明显影响，具有作为生物材料的潜在用途。
