用于检测口腔疣状癌中人乳头瘤病毒的新一代测序分析
Next-generation sequencing analysis for detecting human papillomavirus in oral verrucous carcinoma.
作者信息
Samman Manar, Wood Henry, Conway Caroline, Berri Stefano, Pentenero Monica, Gandolfo Sergio, Cassenti Adele, Cassoni Paola, Al Ajlan Abdulaziz, Barrett A William, Chengot Preetha, MacLennan Kenneth, High Alec S, Rabbitts Pamela
机构信息
Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, United Kingdom.
Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Turin, Italy.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jul;118(1):117-125.e1. doi: 10.1016/j.oooo.2014.03.018. Epub 2014 Apr 12.
OBJECTIVE
The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes.
STUDY DESIGN
We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%.
RESULTS
An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively.
CONCLUSIONS
Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.
目的
口腔疣状癌的病因尚不清楚，人乳头瘤病毒的“参与”仍存在争议。这种不确定性可归因于检测程序的差异以及难以定义诊断“疣状”病变的“金标准”组织学标准。它们的稀缺性也阻碍了研究。我们旨在分析口腔疣状病变中的人乳头瘤病毒（HPV）亚型基因组。
研究设计
我们使用下一代测序技术检测乳头瘤病毒序列，鉴定亚型并计算病毒载量。我们共鉴定出78例口腔疣状病例（62例癌和16例增生）。从所有病例中提取DNA，并在2.5%至13%的覆盖度下进行测序。
结果
在78例病例中，1例癌和1例增生中检测到HPV-16序列，1例癌中检测到HPV-2序列，病毒载量分别为每细胞2.24、8.16和0.33个病毒基因组。
结论
我们的结果表明，没有确凿证据表明人乳头瘤病毒参与口腔疣状癌或增生。
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