Strillacci M G, Frigo E, Canavesi F, Ungar Y, Schiavini F, Zaniboni L, Reghenzani L, Cozzi M C, Samoré A B, Kashi Y, Shimoni E, Tal-Stein R, Soller M, Lipkin E, Bagnato A
Department of Health, Animal Science and Food Safety (VESPA), University of Milan, Via Celoria 10, 20133, Milan, Italy.
Anim Genet. 2014 Aug;45(4):485-99. doi: 10.1111/age.12174. Epub 2014 Jun 9.
A selective DNA pooling approach was applied to identify QTL for conjugated linoleic acid (CLA), vaccenic acid (VA) and Δ(9) -desaturase (D9D) milk content in Italian Brown Swiss dairy cattle. Milk samples from 60 animals with higher values (after correction for environmental factors) and 60 animals with lower values for each of these traits from each of five half-sib families were pooled separately. The pools were genotyped using the Illumina BovineSNP50 BeadChip. Sire allele frequencies were compared between high and low tails at the sire and marker level for SNPs for which the sires were heterozygous. An r procedure was implemented to perform data analysis in a selective DNA pooling design. A correction for multiple tests was applied using the proportion of false positives among all test results. BTA 19 showed the largest number of markers in association with CLA. Associations between SNPs and the VA and Δ(9) -desaturase traits were found on several chromosomes. A bioinformatics survey identified genes with an important role in pathways for milk fat and fatty acids metabolism within 1 Mb of SNP markers associated with fatty acids contents.
采用选择性DNA池化方法来鉴定意大利褐瑞士奶牛中共轭亚油酸（CLA）、反式vaccenic酸（VA）和Δ(9) -去饱和酶（D9D）乳含量的数量性状基因座（QTL）。从五个半同胞家系中，分别采集了60头这些性状值较高（校正环境因素后）的动物和60头性状值较低的动物的乳样。使用Illumina BovineSNP50基因芯片对这些样本池进行基因分型。对于父本杂合的单核苷酸多态性（SNP），在父本和标记水平上比较高尾和低尾之间的父本等位基因频率。实施了一个r程序，以在选择性DNA池化设计中进行数据分析。使用所有测试结果中假阳性的比例对多重测试进行校正。19号牛染色体（BTA 19）显示与CLA相关的标记数量最多。在几条染色体上发现了SNP与VA和Δ(9) -去饱和酶性状之间的关联。一项生物信息学调查在与脂肪酸含量相关的SNP标记的1兆碱基范围内，鉴定出在乳脂肪和脂肪酸代谢途径中起重要作用的基因。
