Marchioro Silvana B, Sácristan Rubén Del Pozo, Michiels Annelies, Haesebrouck Freddy, Conceição Fabricio R, Dellagostin Odir A, Maes Dominiek
Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium; Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vaccine. 2014 Aug 6;32(36):4689-94. doi: 10.1016/j.vaccine.2014.05.072. Epub 2014 Jun 5.
A recombinant chimaeric protein containing three Mycoplasma hyopneumoniae antigens (C-terminal portion of P97, heat shock protein P42, and NrdF) fused to an adjuvant, the B subunit of heat-labile enterotoxin of Escherichia coli (LTB), was used to immunize pigs against enzootic pneumonia. The systemic and local immune responses, as well as the efficacy of the chimaeric protein in inducing protection against experimental M. hyopneumoniae infection were evaluated. In total, 60 male piglets, purchased from a M. hyopneumoniae-free herd, at 4 weeks of age were randomly allocated to six different experimental groups of 10 animals each: recombinant chimaeric protein by intramuscular (IM) (1) or intranasal (IN) (2) administration, commercial bacterin by IM administration (3), and the adjuvant LTB by IM (4, control group A) or IN (5, control group B) administration. All groups were immunized at 24 and 38 days of age and challenged at 52 days of age. The sixth group that was not challenged was used as the negative control (IN [n=5] or IM [n=5] administration of the LTB adjuvant). Compared with the non-challenged group, administration of the chimaeric protein induced significant (P<0.05) IgG and IgA responses against all individual antigens present in the chimaera, but it could not confer a significant protection against M. hyopneumoniae infection in pigs. This lack of effectiveness points towards the need for further studies to improve the efficacy of this subunit-based vaccine approach.
一种重组嵌合蛋白，包含三种猪肺炎支原体抗原（P97的C末端部分、热休克蛋白P42和NrdF），与一种佐剂——大肠杆菌不耐热肠毒素的B亚基（LTB）融合，用于免疫猪以预防地方性肺炎。评估了全身和局部免疫反应，以及该嵌合蛋白在诱导针对实验性猪肺炎支原体感染的保护作用方面的效果。总共60头4周龄从无猪肺炎支原体猪群购买的雄性仔猪被随机分配到六个不同的实验组，每组10只动物：通过肌肉注射（IM）（1组）或鼻内（IN）（2组）给予重组嵌合蛋白，通过IM给予商业菌苗（3组），以及通过IM（4组，对照组A）或IN（5组，对照组B）给予佐剂LTB。所有组在24日龄和38日龄时进行免疫，并在52日龄时进行攻毒。未攻毒的第六组用作阴性对照（通过IN [n = 5]或IM [n = 5]给予LTB佐剂）。与未攻毒组相比，给予嵌合蛋白诱导了针对嵌合体中所有单个抗原的显著（P<0.05）IgG和IgA反应，但它不能为猪提供针对猪肺炎支原体感染的显著保护。这种缺乏有效性表明需要进一步研究以提高这种基于亚单位的疫苗方法的效力。
