Djordjevic S P, Eamens G J, Romalis L F, Nicholls P J, Taylor V, Chin J
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales.
Aust Vet J. 1997 Jul;75(7):504-11. doi: 10.1111/j.1751-0813.1997.tb14383.x.
To investigate the protective efficacy of a pool of denatured membrane protein antigens of Mycoplasma hyopneumoniae (J strain) in the molecular size range 70 to 85 kDa (F3 antigen) in combination with adjuvants for pigs challenged with M hyopneumoniae.
A vaccine efficacy experiment with assessment of serum and respiratory tract antibody responses.
F3 antigens were emulsified with five different adjuvants. To groups of three pigs per vaccine, four vaccines were given by intramuscular injection, and two vaccines, including one of those given intramuscularly, were given by intraperitoneal injection.
Compared to six unvaccinated pigs, animals vaccinated with F3 antigen displayed significantly reduced pneumonia (54% reduction in mean lung score) following experimental challenge. Analysis of post-vaccination, pre-challenge IgG and IgA ELISA antibody absorbances in serum and respiratory tract washings revealed no correlation with lung score. Six weeks after challenge, pigs previously vaccinated intramuscularly mostly demonstrated greater IgG and IgA responses in respiratory tract washings, and greater IgG serum antibody responses, than those vaccinated by intraperitoneal injection.
Pigs vaccinated with M hyponeumoniae antigens in the molecular size range of 70 to 85 kDa showed a significant reduction in lung lesions compared with unvaccinated control animals after experimental challenge. IgG and IgA antibody concentrations in serum and respiratory tract washings after vaccination do not provide a useful prognostic indicator of protection from enzootic pneumonia.
研究猪肺炎支原体（J株）70至85 kDa分子大小范围内的一组变性膜蛋白抗原（F3抗原）与佐剂联合使用对受猪肺炎支原体攻击的猪的保护效果。
一项评估血清和呼吸道抗体反应的疫苗效力试验。
将F3抗原与五种不同佐剂乳化。每种疫苗给三组猪注射，四种疫苗通过肌肉注射给药，两种疫苗，包括其中一种肌肉注射的疫苗，通过腹腔注射给药。
与六只未接种疫苗的猪相比，接种F3抗原的动物在实验性攻毒后肺炎显著减轻（平均肺评分降低54%）。对疫苗接种后、攻毒前血清和呼吸道冲洗液中IgG和IgA ELISA抗体吸光度的分析显示与肺评分无相关性。攻毒六周后，先前肌肉注射疫苗的猪在呼吸道冲洗液中大多表现出比腹腔注射疫苗的猪更强的IgG和IgA反应，以及更强的IgG血清抗体反应。
与未接种疫苗的对照动物相比，接种70至85 kDa分子大小范围内猪肺炎支原体抗原的猪在实验性攻毒后肺部病变显著减少。接种疫苗后血清和呼吸道冲洗液中的IgG和IgA抗体浓度不能作为预防地方性肺炎保护效果的有用预后指标。
