Irshad M, Gandhi B M, Acharya S K, Joshi Y K, Tandon B N
Department of Gastroenterology & Human Nutrition, All-India Institute of Medical Sciences, New Delhi.
J Gastroenterol Hepatol. 1989 Jan-Feb;4(1):25-32. doi: 10.1111/j.1440-1746.1989.tb00803.x.
The anti-pre-S antibody in the samples of sera from normal healthy persons and patients with different clinical types of liver diseases due to hepatitis B virus (HBV) infection was detected by a newly established enzyme-linked immunosorbent assay technique. This test is a blocking assay where anti-pre-S antibody in the patient's serum blocks subsequent addition of horse radish peroxidase-labelled polymerized human serum albumin (pHSA) to the pHSA-receptor site of HBsAg molecules fixed on a solid surface. Anti-pre-S activity was not detected in any from 95 healthy persons who were negative for all HBV-markers or from 105 healthy HBV carriers. In 12 sera from HBV vaccine recipients, anti-pre-S activity was noted in higher proportions compared with anti-HBs, after both the second and third doses of vaccine. Anti-pre-S activity was detected in small proportions of HBsAg positive sera from acute viral hepatitis (4.2%) and chronic active hepatitis (10%). In subacute viral hepatitis patients, the anti-pre-S antibody was totally absent. However, anti-pre-S activity was recorded in high proportions of HBsAg-positive sera from patients with cirrhosis of liver (57.2%) and fulminant hepatitis (41.6%). The anti-pre-S antibodies were assumed to be implicated in the clearance of HBV particles from circulation without causing tissue damage.
采用新建立的酶联免疫吸附测定技术，检测了正常健康人和不同临床类型乙肝病毒（HBV）感染肝病患者血清样本中的抗前S抗体。该检测为阻断试验，即患者血清中的抗前S抗体可阻断随后将辣根过氧化物酶标记的聚合人血清白蛋白（pHSA）添加至固定在固体表面的HBsAg分子的pHSA受体位点。在95名所有HBV标志物均为阴性的健康人和105名健康HBV携带者中，均未检测到抗前S活性。在12名接种HBV疫苗者的血清中，在接种第二剂和第三剂疫苗后，与抗-HBs相比，检测到抗前S活性的比例更高。在急性病毒性肝炎（4.2%）和慢性活动性肝炎（10%）患者的小部分HBsAg阳性血清中检测到抗前S活性。在亚急性病毒性肝炎患者中，完全没有抗前S抗体。然而，在肝硬化患者（57.2%）和暴发性肝炎患者（41.6%）的大部分HBsAg阳性血清中记录到抗前S活性。抗前S抗体被认为与从循环中清除HBV颗粒有关，且不会造成组织损伤。
