Irshad M, Gandhi B M, Acharya S K, Joshi Y K, Tandon B N
J Immunol Methods. 1987 Feb 11;96(2):211-7. doi: 10.1016/0022-1759(87)90316-4.
A simple and specific enzyme-linked immunosorbent assay (ELISA) has been developed to detect circulating IgG and IgM anti-idiotypic antibodies directed against anti-HBs molecules using 96-well polyvinyl microtitre plates as the solid phase and HRPO-labelled goat anti-HBs as conjugate. Anti-idiotype reactions were observed in the supernatant portion after precipitation of immune complexes from sera with polyethylene glycol 6000 (PEG). Both IgG and IgM with anti-idiotype activity were detected concurrently in HBsAg-positive sera from HBV-infected patients and asymptomatic HBV carriers. Anti-idiotype activity was absent in HBsAg-negative sera from healthy persons, and in patients with non-A, non-B hepatitis and viral hepatitis A. However, such antibodies could be demonstrated in the sera of two out of eight HBsAg vaccine recipients negative for anti-HBs but in none of 11 recipients positive for anti-HBs after receiving a booster immunising dose of HBsAg vaccine. Those sera showing positive anti-idiotype reactions were free from rheumatoid factor and HBsAg/IgM or HBsAg/IgG complex activity. An analysis of anti-idiotype positive sera for anti-HBs, HBeAg and HBV-specific DNA-polymerase activity demonstrated these markers in 20%, 30% and 60% of cases, respectively. The presence of anti-idiotypic antibodies was presumed to permit a more active multiplication of hepatitis B virus.
已开发出一种简单且特异的酶联免疫吸附测定法（ELISA），以96孔聚乙烯微量滴定板为固相，用辣根过氧化物酶（HRPO）标记的山羊抗-HBs作为结合物，来检测针对抗-HBs分子的循环IgG和IgM抗独特型抗体。在用聚乙二醇6000（PEG）从血清中沉淀免疫复合物后，在上清液部分观察到抗独特型反应。在乙肝病毒（HBV）感染患者和无症状HBV携带者的HBsAg阳性血清中同时检测到具有抗独特型活性的IgG和IgM。健康人的HBsAg阴性血清、非甲非乙型肝炎患者和甲型病毒性肝炎患者的血清中均无抗独特型活性。然而，在8名抗-HBs阴性的HBsAg疫苗接种者的血清中，有2人的血清可检测到此类抗体，但在11名接种HBsAg疫苗加强免疫剂量后抗-HBs阳性的接种者中，无一例检测到此类抗体。那些抗独特型反应呈阳性的血清中无类风湿因子以及HBsAg/IgM或HBsAg/IgG复合物活性。对抗独特型阳性血清进行抗-HBs、HBeAg和HBV特异性DNA聚合酶活性分析，结果显示这些标志物的阳性率分别为20%、30%和60%。据推测，抗独特型抗体的存在会使乙型肝炎病毒的增殖更为活跃。
