Pang Min, He Shujian, Wang Lu, Cao Xinmin, Cao Lili, Jiang Shaotong
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei, 230009, P. R. China.
Food Funct. 2014 Aug;5(8):1763-70. doi: 10.1039/c4fo00106k.
This study was designed to investigate physicochemical characterization of the oil extracted from foxtail millet bran (FMBO), and the antioxidant and hepatoprotective effects against acute ethanol-induced hepatic injury in mice. GC-MS analysis revealed that unsaturated fatty acids (UFAs) account for 83.76% of the total fatty acids; in particular, the linoleic acid (C18:2) is the predominant polyunsaturated fatty acid (PUFA), and the compounds of squalene and six phytosterols (or phytostanols) were identified in unsaponifiable matter of FMBO. The antioxidant activity examination of FMBO in vitro showed highly ferric-reducing antioxidant power and scavenging effects against DPPH· and HO· radicals. Furthermore, the protective effect of FMBO against acute hepatic injuries induced by ethanol was verified in mice. In this, intragastric administration with different dosages of FMBO in mice ahead of acute ethanol administration could observably antagonize the ethanol-induced increases in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and the hepatic malondialdehyde (MDA) levels, respectively, along with enhanced hepatic superoxide dismutase (SOD) levels relative to the control. Hepatic histological changes were also observed and confirmed that FMBO is capable of attenuating ethanol-induced hepatic injury.
本研究旨在探究从谷子麸皮中提取的油（FMBO）的理化特性，以及其对小鼠急性乙醇诱导的肝损伤的抗氧化和肝保护作用。气相色谱-质谱联用（GC-MS）分析显示，不饱和脂肪酸（UFA）占总脂肪酸的83.76%；特别是，亚油酸（C18:2）是主要的多不饱和脂肪酸（PUFA），并且在FMBO的不皂化物中鉴定出了角鲨烯和六种植物甾醇（或植物甾烷醇）化合物。FMBO的体外抗氧化活性检测显示出高的铁还原抗氧化能力以及对DPPH·和HO·自由基的清除作用。此外，FMBO对小鼠乙醇诱导的急性肝损伤的保护作用得到了验证。在此实验中，在急性给予乙醇之前给小鼠灌胃不同剂量的FMBO，可分别显著拮抗乙醇诱导的血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、甘油三酯（TG）和肝丙二醛（MDA）水平的升高，同时相对于对照组，肝超氧化物歧化酶（SOD）水平增强。还观察了肝脏组织学变化，并证实FMBO能够减轻乙醇诱导的肝损伤。
