Zhou Yuan, Zeng Kewu, Zhang Jiayu, Li Ning, Chai Xingyun, Jiang Yong, Tu Pengfei
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China.
Fitoterapia. 2014 Sep;97:98-104. doi: 10.1016/j.fitote.2014.05.020. Epub 2014 Jun 6.
Five new triterpene saponins, Ilexpublesnins N-R (1-5), along with seven known analogs were isolated from the root of Ilex pubescens. Their structures were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR experiments. Ilexpublesnin N (1) possessed a rare 20-hydroxyursolic acid scaffold from natural resource. These compounds were evaluated in vitro for their cytotoxic effects on human cancer cell lines HepG2, HLE, BEL7402, BEL7403, BEL7405, MCF-7, HeLa. Among them, only compounds 5 and 10 showed cytotoxic potentiality against BEl-7403 and HEL cell lines [inhibition (%): 35.38 and 45.12, respectively].
从毛冬青根中分离得到5个新的三萜皂苷，毛冬青皂苷N - R（1 - 5），以及7个已知类似物。通过广泛的光谱分析，包括一维和二维核磁共振实验，阐明了它们的结构。毛冬青皂苷N（1）具有一种罕见的来自自然资源的20 - 羟基熊果酸骨架。对这些化合物进行了体外实验，评估它们对人癌细胞系HepG2、HLE、BEL7402、BEL7403、BEL7405、MCF - 7、HeLa的细胞毒性作用。其中，只有化合物5和10对BEl - 7403和HEL细胞系显示出细胞毒性潜力[抑制率（%）：分别为35.38和45.12]。
