Singh Garvita, Babele Piyoosh K, Kumar Ashok, Srivastava Anup, Sinha Rajeshwar P, Tyagi Madhu B
School of Biotechnology, Banaras Hindu University, Varanasi 221005, India.
School of Biotechnology, Banaras Hindu University, Varanasi 221005, India.
J Photochem Photobiol B. 2014 Sep 5;138:55-62. doi: 10.1016/j.jphotobiol.2014.04.030. Epub 2014 May 21.
In the present work, we describe a cheap, unexplored and simple procedure for the synthesis of zinc oxide nanoparticles (ZnONPs) using the cell extract of the cyanobacterium, Anabaena strain L31. An attempt was also made to conjugate synthesized ZnONPs with a UV-absorbing water soluble compound shinorine. UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infra-red (FTIR) spectroscopy, transmission electron microscopy (TEM) and TEM-selected area electron diffraction (SAED) analyses were made to elucidate the formation and characterization of ZnONPs and ZnONPs-shinorine conjugate. The synthesized ZnONPs were characterized by a sharp peak at 370 nm in UV-vis spectrum. TEM images showed the formation of spherical shaped nanoparticles with an average size of 80 nm. Results of selective area electron diffraction (SAED) pattern showed a set of rings which suggested uniform shape with hexagonal structure of ZnONPs. XRD spectra confirmed the crystalline structure of particles. Conjugation of ZnONPs with shinorine was successfully achieved at pH 7.0 and 10mM concentration of shinorine. The conjugate showed a zeta potential value of -3.75 mV as compared to +30.25 mV of ZnONPs. The change in zeta potential value of ZnONPs-shinorine conjugate was attributed to the changes in the surface functionalities after conjugation. The generation of in vivo reactive oxygen species (ROS) by Anabaena strain L31 with treatment of ZnONPs-shinorine conjugate showed approximately 75% less ROS generation as compared to ZnONPs. Properties exhibited by the ZnONPs-shinorine conjugate suggest that it may be used as a potential agent in developing environmental-friendly sunscreen filters of biological origin.
在本研究中，我们描述了一种利用蓝藻鱼腥藻L31菌株的细胞提取物合成氧化锌纳米颗粒（ZnONPs）的廉价、未被探索且简单的方法。我们还尝试将合成的ZnONPs与一种吸收紫外线的水溶性化合物紫菜碱进行共轭。通过紫外可见光谱、X射线衍射（XRD）、傅里叶变换红外（FTIR）光谱、透射电子显微镜（TEM）和TEM选区电子衍射（SAED）分析来阐明ZnONPs和ZnONPs-紫菜碱共轭物的形成及特性。合成的ZnONPs在紫外可见光谱中于370 nm处有一个尖锐峰。TEM图像显示形成了平均尺寸为80 nm的球形纳米颗粒。选区电子衍射（SAED）图案的结果显示出一组环，表明ZnONPs具有六边形结构且形状均匀。XRD光谱证实了颗粒的晶体结构。在pH 7.0和紫菜碱浓度为10 mM的条件下，成功实现了ZnONPs与紫菜碱的共轭。与ZnONPs的+30.25 mV相比，共轭物的zeta电位值为-3.75 mV。ZnONPs-紫菜碱共轭物zeta电位值的变化归因于共轭后表面官能团的变化。用ZnONPs-紫菜碱共轭物处理的鱼腥藻L31菌株产生的体内活性氧（ROS）与ZnONPs相比减少了约75%。ZnONPs-紫菜碱共轭物所表现出的特性表明，它可能作为一种潜在试剂用于开发具有生物来源的环保型防晒滤光剂。
