藻衍生氧化铜纳米粒子的毒理学评估及其在生物医学和环境中的应用。

Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles for its biomedical and environmental applications.

机构信息

Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India.

Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110025, India.

出版信息

Sci Rep. 2023 Apr 17;13(1):6246. doi: 10.1038/s41598-023-33360-3.

Driven by the need to biosynthesized alternate biomedical agents to prevent and treat infection, copper oxide nanoparticles (CuONPs) have surfaced as a promising avenue. Cyanobacteria-derived synthesis of CuONPs is of substantive interest as it offers an eco-friendly, cost-effective, and biocompatible route. In the present study biosynthesized CuONPs were characterized and investigated regarding their toxicity. Morphological analysis using TEM, SEM and AFM showed the spherical particle size of 20.7 nm with 96% copper that confirmed the purity of CuONPs. Biogenic CuONPs with IC value of 64.6 µg ml showed 90% scavenging of free radicals in superoxide radical scavenging assay. CuONPs showed enhanced anti-inflammatory activity by 86% of protein denaturation with IC value of 89.9 µg ml. Biogenic CuONPs exhibited significant toxicity against bacterial strains with lowest MIC value of 62.5 µg ml for B. cereus and fungal strain with a MIC value of 125 µg ml for C. albicans. In addition CuONPs demonstrated a high degree of synergistic interaction when combined with standard drugs. CuONPs exhibited significant cytotoxicity against non-small cell lung cancer with an IC value of 100.8 µg ml for A549 and 88.3 µg ml for the H1299 cell line with apoptotic activities. Furthermore, biogenic CuONPs was evaluated for their photocatalytic degradation potential against methylene blue dye and were able to removed 94% dye in 90 min. Free radical scavenging analysis suggested that CuONPs assisted dye degradation was mainly induced by hydroxide radicals. Biogenic CuONPs appears as an eco-friendly and cost effective photocatalyst for the treatment of wastewater contaminated with synthetic dyes that poses threat to aquatic biota and human health. The present study highlighted the blend of biomedical and photocatalytic potential of Phormidium derived CuONPs as an attractive approach for future applications in nanomedicine and bioremediation.

受合成替代生物医学制剂以预防和治疗感染的需求驱动，氧化铜纳米粒子 (CuONPs) 成为一个很有前途的途径。由于其提供了一种环保、经济高效且生物相容的途径，因此从蓝藻中合成 CuONPs 具有实质性的意义。在本研究中，我们对生物合成的 CuONPs 进行了表征，并研究了其毒性。使用 TEM、SEM 和 AFM 进行形态分析表明，CuONPs 的球形粒径为 20.7nm，铜纯度为 96%，这证实了 CuONPs 的纯度。生物合成的 CuONPs 的 IC 值为 64.6µg ml，在超氧自由基清除试验中显示出 90%的自由基清除率。CuONPs 对蛋白质变性的抗炎活性增强了 86%，IC 值为 89.9µg ml。生物合成的 CuONPs 对细菌菌株表现出显著的毒性，最低 MIC 值为 62.5µg ml 对蜡样芽孢杆菌和最低 MIC 值为 125µg ml 对白色念珠菌。此外，当与标准药物联合使用时，CuONPs 表现出高度的协同相互作用。CuONPs 对非小细胞肺癌表现出显著的细胞毒性，A549 的 IC 值为 100.8µg ml，H1299 细胞系的 IC 值为 88.3µg ml，具有凋亡活性。此外，还评估了生物合成的 CuONPs 对亚甲基蓝染料的光催化降解潜力，能够在 90 分钟内去除 94%的染料。自由基清除分析表明，CuONPs 辅助的染料降解主要是由氢氧自由基引起的。生物合成的 CuONPs 似乎是一种环保且经济高效的光催化剂，可用于处理对水生生物和人类健康构成威胁的合成染料污染的废水。本研究强调了源自 Phormidium 的 CuONPs 的生物医学和光催化潜力的融合，作为未来在纳米医学和生物修复中应用的有吸引力的方法。