Naghibi Farzaneh, Khalaj Amir, Mosaddegh Mahmoud, Malekmohamadi Maryam, Hamzeloo-Moghadam Maryam
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, No. 8 Shams Alley, Vali-e-Asr Street, 1516745811, Tehran, Iran.
J Ethnopharmacol. 2014 Aug 8;155(1):230-9. doi: 10.1016/j.jep.2014.05.025. Epub 2014 Jun 6.
By studying the Iranian Traditional Medicine (ITM) Pharmacopoeia, we have collected information about medicinal plants which had been used to manage cancer-like disorders over eight hundred years, from medieval to the early modern era. Exploring the ITM herbal knowledge, the selected species have been subjected to MTT assay for examining their in vitro cyototoxic activity.
Cancer was reviewed through the ITM and some terminologies were acquired. Five ITM Pharmacopoeia in Arabic and Persian languages from 10th to 18th century AD were explored. These Pharmacopoeia contained the ITM plants which were used to cure cancer and cancer-like diseases. The ITM names were matched with the scientific names. Then the medicinal plants were collected, authenticated and were evaluated for cytotoxic activity using MTT assay, against MCF-7, HepG-2, A-549 and HT-29 cell lines. Finally, the apoptosis induction ability of the most cytotoxic medicinal plant was investigated by activated caspase 3 inspection in MCF-7 cell line.
Six species of the evaluated medicinal plants revealed cytotoxic activity. The most cytotoxicity was observed in Tanacetum polycephalum subsp. argyrophyllum (K.Koch) Podlech with IC50 values of 28.3 μg mL(-1), 53.9 μg mL(-1) and 43.3 μg mL(-1) against MCF-7, A-549 and HT-29 cell lines, respectively. Caspase 3 activation was also observed in MCF-7 cells by Tanacetum polycephalum subsp. argyrophyllum. This is the first time that the cytotoxic activity of this species has been reported.
In the present study, some reliable references of ITM have been introduced and though many technical difficulties, linguistic problems and some other hindrances were encountered during the study, using traditional medicine texts for medicinal plant selection could be considered as a helpful starting point in the field of cancer drug discovery.
通过研究伊朗传统医学（ITM）药典，我们收集了从中世纪到近代早期八百多年来用于治疗类似癌症病症的药用植物信息。探索ITM草药知识后，对所选物种进行MTT分析以检测其体外细胞毒性活性。
通过ITM对癌症进行了综述并获取了一些术语。查阅了公元10世纪至18世纪的五本阿拉伯语和波斯语的ITM药典。这些药典包含用于治疗癌症和类似癌症疾病的ITM植物。将ITM名称与科学名称进行匹配。然后收集药用植物，进行鉴定，并使用MTT分析针对MCF-7、HepG-2、A-549和HT-29细胞系评估其细胞毒性活性。最后，通过检测MCF-7细胞系中活化的半胱天冬酶3来研究最具细胞毒性的药用植物的凋亡诱导能力。
六种评估的药用植物显示出细胞毒性活性。在多裂菊蒿银叶亚种（K.Koch）Podlech中观察到最强的细胞毒性，其对MCF-7、A-549和HT-29细胞系的IC50值分别为28.3μg/mL、53.9μg/mL和43.3μg/mL。多裂菊蒿银叶亚种在MCF-7细胞中也观察到半胱天冬酶3的激活。这是首次报道该物种的细胞毒性活性。
在本研究中，引入了一些可靠的ITM参考文献，尽管在研究过程中遇到了许多技术困难、语言问题和其他一些障碍，但利用传统医学文本进行药用植物选择可被视为癌症药物发现领域的一个有益起点。
