硫辛酸对邻苯二甲酸二丁酯诱导的大鼠睾丸毒性的影响。
Influence of lipoic acid on testicular toxicity induced by bi-n-butyl phthalate in rats.
作者信息
El-Beshbishy Hesham A, Mariah Reham A, Al-Azhary Nevin M, Aly Hamdy A A, Ozbak Hani A, Baghdadi Hussam H
机构信息
Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia; Biochemistry Department, Al-Azhar University, Nasr City, Cairo 11751, Egypt.
Biochemistry and Molecular Medicine Department, College of Medicine, Taibah University, Saudi Arabia; Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.
出版信息
Food Chem Toxicol. 2014 Sep;71:26-32. doi: 10.1016/j.fct.2014.05.024. Epub 2014 Jun 6.
Bi-n-butyl phthalate (BNBP) is an environmental pollutant. The aim of this study was to evaluate the protective effect of lipoic acid (LA) against testicular dysfunction associated with the intake of to BNBP- intoxicated rats. Adult male Wistar rats were divided into 4 groups of 6 animals each, and received medication orally for 14 days. Group I rats received 0.5 ml corn oil. Group II rats received LA (20 mg/kg B.W./day). Group III rats received BNBP (250 mg/kg B.W./day). Group IV rats received LA 24h prior to BNBP intake. Testes weight, cauda sperm count and sperm motility were decreased significantly by 18.15%, 13.83% and 13.5%, respectively, after BNBP treatment. Significant increase by 12.1%, 10.20% and 11.51%, respectively, was observed in LA-BNBP rats. Significant increase by 1.53%, 1.5% and 1.8%, for serum follicle stimulating hormone, testosterone and total antioxidant status, respectively, were observed in LA-BNBP rats. Testicular lipid peroxides and lactate dehydrogenase enzyme were significantly decreased by 1.5 and 1.6 folds, respectively, in LA-BNBP rats were decreased after BNBP treatment. Testicular superoxide dismutase, catalase and glutathione reductase enzymes were significantly increased in LA-BNBP rats. LA-BNBP rats, decreased the damage to seminiferous tubules produced by BNBP intake. In conclusion, LA mitigated BNBP-induced testicular toxicity through antioxidant mechanism and by direct free radical scavenging activity.
邻苯二甲酸二正丁酯（BNBP）是一种环境污染物。本研究的目的是评估硫辛酸（LA）对与摄入BNBP的中毒大鼠相关的睾丸功能障碍的保护作用。成年雄性Wistar大鼠被分成4组，每组6只动物，并口服给药14天。第一组大鼠接受0.5毫升玉米油。第二组大鼠接受LA（20毫克/千克体重/天）。第三组大鼠接受BNBP（250毫克/千克体重/天）。第四组大鼠在摄入BNBP前24小时接受LA。在BNBP处理后，睾丸重量、附睾精子计数和精子活力分别显著降低了18.15%、13.83%和13.5%。在LA-BNBP组大鼠中分别观察到显著增加了12.1%、10.20%和11.51%。在LA-BNBP组大鼠中，血清促卵泡激素、睾酮和总抗氧化状态分别显著增加了1.53%、1.5%和1.8%。在LA-BNBP组大鼠中，睾丸脂质过氧化物和乳酸脱氢酶分别显著降低了1.5倍和1.6倍，在BNBP处理后降低。在LA-BNBP组大鼠中，睾丸超氧化物歧化酶、过氧化氢酶和谷胱甘肽还原酶显著增加。LA-BNBP组大鼠减少了因摄入BNBP对生精小管产生的损伤。总之，LA通过抗氧化机制和直接的自由基清除活性减轻了BNBP诱导的睾丸毒性。
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