Aly Hamdy Aa, Hassan Memy H, El-Beshbishy Hesham A, Alahdal Abdulrahman M, Osman Abdel-Moneim M
Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
Toxicol Ind Health. 2016 Aug;32(8):1467-1477. doi: 10.1177/0748233714566877. Epub 2015 Jan 22.
Phthalates are abundantly produced plasticizers, and dibutyl phthalate (DBP) is the most widely used derivative in various consumer products and medical devices. This study was conducted to further explore the potential testicular toxicity of DBP in adult rats and to elucidate the underlying mechanisms. Adult male albino rats were treated orally with DBP at doses of 0, 200, 400, or 600 mg/kg/day for 15 consecutive days. Testicular weight, sperm count, and motility were significantly decreased. Treatment with DBP decreased serum follicle-stimulating hormone and testosterone levels and testicular lactate dehydrogenase activity. DBP treatment also decreased serum total antioxidant capacity and the activities of the testicular antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione reductase. Further, DBP treatment provoked degeneration with absence of spermatogenesis and sperms and necrosis in some of seminiferous tubules. These results indicated that oxidative stress and subsequent decrease in testosterone secretion were the potential underlying mechanism of DBP-induced testicular toxicity.
邻苯二甲酸盐是大量生产的增塑剂，邻苯二甲酸二丁酯（DBP）是在各种消费品和医疗设备中使用最广泛的衍生物。本研究旨在进一步探讨DBP对成年大鼠潜在的睾丸毒性，并阐明其潜在机制。成年雄性白化大鼠连续15天口服给予0、200、400或600mg/kg/天剂量的DBP。睾丸重量、精子计数和活力显著降低。DBP处理降低了血清促卵泡激素和睾酮水平以及睾丸乳酸脱氢酶活性。DBP处理还降低了血清总抗氧化能力以及睾丸抗氧化酶如超氧化物歧化酶、过氧化氢酶和谷胱甘肽还原酶的活性。此外，DBP处理引发了生精细胞和精子缺失以及一些生精小管坏死的退化现象。这些结果表明氧化应激以及随后睾酮分泌减少是DBP诱导睾丸毒性的潜在机制。
