Zhang Wenqiang, Yang Ye, Liu Wei, Chen Qian, Wang Huarong, Wang Xiao, Zhang Yanhao, Zhang Meijia, Xia Guoliang
State Key Laboratory of Agro-biotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
J Cell Physiol. 2015 Jan;230(1):71-81. doi: 10.1002/jcp.24682.
Recent studies have shown that C-type natriuretic peptide (CNP) serves as a key control system during mouse oocyte maturation. We used pig models (in vitro and in vivo) to explore the role played by the natriuretic peptide family in porcine oocyte maturation. We reported the expression and location of natriuretic peptide system in different stages of porcine antral follicles. Atrial natriuretic peptide (ANP) and CNP were expressed primarily in granulosa cells, whereas brain natriuretic peptide (BNP) and natriuretic peptide receptor-B (NPRB) receptor were expressed in granulosa cells (both cumulus and mural granulosa cells) and thecal internal cells, and the natriuretic peptide receptor-A (NPRA) receptor predominantly in thecal cells. Upon in vitro culture, BNP and CNP maintained meiotic arrest of oocytes associated with cumulus cells. The expression levels of BNP, CNP, and the NPRB receptor increased upon treatment of prepubertal gilts with pregnant mare's serum gonadotropin and decreased upon subsequent human chorionic gonadotropin injection. Such dynamic changes in the expression of natriuretic peptides and their receptor paralleled the proportions of oocytes exhibiting nuclear maturation in vivo. These data indicated that BNP and CNP co-contributed to maintaining porcine meiotic arrest under physiological condition and lutenizing hormone (LH) relieved this inhibitory effect by decreasing the expression levels of BNP and CNP in vivo. Our present work, combined with previous data, improved the understanding of the oocyte meiotic arrest mechanisms and further revealed that natriuretic peptides serve as oocyte maturation inhibitor (OMI) to inhibit oocyte maturation in mammals.
最近的研究表明，C型利钠肽（CNP）在小鼠卵母细胞成熟过程中起关键控制系统的作用。我们使用猪模型（体外和体内）来探究利钠肽家族在猪卵母细胞成熟中所起的作用。我们报道了利钠肽系统在猪窦卵泡不同阶段的表达和定位。心房利钠肽（ANP）和CNP主要在颗粒细胞中表达，而脑利钠肽（BNP）和利钠肽受体-B（NPRB）受体在颗粒细胞（卵丘和壁层颗粒细胞）和卵泡内膜细胞中表达，利钠肽受体-A（NPRA）受体主要在卵泡膜细胞中表达。体外培养时，BNP和CNP维持与卵丘细胞相关的卵母细胞减数分裂阻滞。用孕马血清促性腺激素处理青春期前小母猪后，BNP、CNP和NPRB受体的表达水平升高，随后注射人绒毛膜促性腺激素后表达水平降低。利钠肽及其受体表达的这种动态变化与体内表现出核成熟的卵母细胞比例平行。这些数据表明，BNP和CNP在生理条件下共同有助于维持猪的减数分裂阻滞，促黄体生成素（LH）通过降低体内BNP和CNP的表达水平来解除这种抑制作用。我们目前的工作与先前的数据相结合，增进了对卵母细胞减数分裂阻滞机制的理解，并进一步揭示利钠肽作为卵母细胞成熟抑制剂（OMI）在哺乳动物中抑制卵母细胞成熟。
