Bonina L, Delfino D, Costa G B, Arena A
Microbiology Institute, Messina University, Italy.
Boll Ist Sieroter Milan. 1989;68(1):62-6.
Corynebacterium parvum has been used as an anti-tumor agent in both animal experiments and clinical trials; nevertheless it is not well established the mechanism of action, since both T-dependent and T-independent ways of action have been proposed. On the other hand, mononuclear phagocytic system activation cannot be ruled out in the immunomodulation carried out by C. parvum. In the attempt to better understand the effects of C. parvum on macrophage functions, experiments were carried out on tumor-bearing rats with a decline of peritoneal macrophage activities as a result of the tumor development. For this purpose three different preparations of C. parvum were investigated: a) the first preparation was obtained by heat-inactivation of bacteria at 60 degrees C for 1h; b) the second by formalin-treatment of bacteria; c) the third obtained from Burrough Wellcome as a formaline-killed suspension in 0.01% w/v thiomersal saline. Each preparation was used at 7 mg/ml dry weight and administered subcutaneously, three days after tumor grafting. The results showed that all three preparations were capable to hinder the tumor mass growth. Macrophage functions as phagocytosis, intracellular killing, intrinsic and extrinsic anti-Herpes Simplex Virus activities, were restored to normal values by each of the preparations used. Nevertheless, concerning the chemotactic activity a discrepancy was noted among the three preparations, since the heat-inactivated one was able to carried out a complete restoration.
短小棒状杆菌已在动物实验和临床试验中用作抗肿瘤药物；然而，其作用机制尚未完全明确，因为已提出了依赖T细胞和不依赖T细胞的作用方式。另一方面，在短小棒状杆菌进行的免疫调节中不能排除单核吞噬系统的激活。为了更好地了解短小棒状杆菌对巨噬细胞功能的影响，对荷瘤大鼠进行了实验，这些大鼠由于肿瘤发展导致腹腔巨噬细胞活性下降。为此，研究了三种不同的短小棒状杆菌制剂：a）第一种制剂是通过将细菌在60℃加热灭活1小时获得的；b）第二种是通过用福尔马林处理细菌获得的；c）第三种是从百时美施贵宝公司获得的，为在0.01% w/v硫柳汞盐水中的福尔马林灭活悬浮液。每种制剂以7 mg/ml干重使用，并在肿瘤移植后三天皮下给药。结果表明，所有三种制剂都能够抑制肿瘤块的生长。每种使用的制剂都将巨噬细胞的吞噬作用、细胞内杀伤、内在和外在抗单纯疱疹病毒活性等功能恢复到了正常水平。然而，关于趋化活性，三种制剂之间存在差异，因为热灭活的制剂能够实现完全恢复。
