Effect of vaccine prepared from Corynebacterium parvum on the growth of Gardner lymphosarcoma in C3H strain mice.

The present paper discusses the effect of a vaccine prepared with formol-killed Corynebacterium parvum (alias Propionibacterium acnes) on the growth of solid Gardner lymphosarcoma transplanted on C3H (H-2k) mice. The vaccine was injected at various time intervals prior to, simultaneously with, or following transplantation of the tumor to intact mice or mice immunized with 60Co-irradiated Gardner tumor cells. The effectivity was evaluated in terms of numbers of mice surviving without tumor 60-day observation period or prolongation of survival time. Reliable effectivity showed vaccine (0.8 mg dry weight of C.p.) in the mixture with tumor transplant or when introduced 6 days earlier into the site of tumor transplantation. The preventive action of the vaccine was more pronounced in the mice immunized with inactivated tumor. The vaccine applied to mice with a transplanted tumor was ineffective and failed to significantly prolong the survival time even in mice which had previously been immunized with an inactivated tumor.