Palod Pragya Agar, Pandey Shyam S, Hayase Shuji, Singh Vipul
Molecular and Nanoelectronics Research Group (MNRG), Department of Electrical Engineering, Indian Institute of Technology Indore, Pithampur Auto Cluster Limited (PACL) Building, Survey No. 113/2-B, Village Hernia Khedi, Mhow, Indore, Madhya Pradesh, India.
Appl Biochem Biotechnol. 2014 Oct;174(3):1059-72. doi: 10.1007/s12010-014-0988-x. Epub 2014 Jun 10.
In this paper, we report the growth of polypyrrole (PPy) nanotube arrays using template-assisted electrochemical polymerization to fabricate enzymatic glucose biosensors. The PPy nanotubes were grown on platinum-coated alumina membranes (Anodisc™s). By varying the polymerization time during the potentiostatic electropolymerization, the size/diameter of the PPy nanotubes were controlled, leading to changes in the subsequent enzyme immobilization (via physical adsorption). Enzyme electrode thus fabricated resulted in to the optimum sensitivity of 18.6 mA cm(-2) M(-1), a wide range of linear operation (0.25-20 mM) and the lowest detection limit of 0.25 mM glucose concentration for the biosensor with the polymerization time of 40 s. The effect of polymerization duration on the sensitivity has been explained on the basis of porosity and enzyme-loading capacity of polymerized electrodes.
在本文中，我们报道了使用模板辅助电化学聚合方法生长聚吡咯（PPy）纳米管阵列以制造酶促葡萄糖生物传感器。PPy纳米管生长在镀铂的氧化铝膜（Anodisc™）上。通过在恒电位电聚合过程中改变聚合时间，可以控制PPy纳米管的尺寸/直径，从而导致随后酶固定化（通过物理吸附）的变化。由此制备的酶电极对葡萄糖生物传感器而言，在聚合时间为40秒时，可实现18.6 mA cm(-2) M(-1)的最佳灵敏度、0.25 - 20 mM的宽线性操作范围以及0.25 mM葡萄糖浓度的最低检测限。已基于聚合电极的孔隙率和酶负载能力解释了聚合持续时间对灵敏度的影响。
