对一株对甲硝唑具有稳定耐药性的非产毒艰难梭菌菌株的多学科分析。
Multidisciplinary analysis of a nontoxigenic Clostridium difficile strain with stable resistance to metronidazole.
作者信息
Moura Ines, Monot Marc, Tani Chiara, Spigaglia Patrizia, Barbanti Fabrizio, Norais Nathalie, Dupuy Bruno, Bouza Emilio, Mastrantonio Paola
机构信息
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
Laboratoire Pathogenèse des Bactéries Anaérobies, Institut Pasteur, Paris, France.
出版信息
Antimicrob Agents Chemother. 2014 Aug;58(8):4957-60. doi: 10.1128/AAC.02350-14. Epub 2014 Jun 9.
Abstract
Stable resistance to metronidazole in a nontoxigenic Clostridium difficile strain was investigated at both the genomic and proteomic levels. Alterations in the metabolic pathway involving the pyruvate-ferredoxin oxidoreductase were found, suggesting that reduction of metronidazole, required for its activity, may be less efficient in this strain. Proteomic studies also showed a cellular response to oxidative stress.
摘要
在基因组和蛋白质组水平上研究了一株非产毒素艰难梭菌对甲硝唑的稳定耐药性。发现涉及丙酮酸-铁氧还蛋白氧化还原酶的代谢途径发生了改变，这表明该菌株中甲硝唑发挥活性所需的还原过程可能效率较低。蛋白质组学研究还显示了细胞对氧化应激的反应。
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