Hu Lianghai, Boos Karl-Siegfried, Ye Mingliang, Zou Hanfa
Key Laboratory Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China; Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Laboratory of BioSeparation, Institute of Clinical Chemistry, University Hospital Grosshadern, D-81366 Munich, Germany.
Talanta. 2014 Sep;127:191-5. doi: 10.1016/j.talanta.2014.04.011. Epub 2014 Apr 16.
The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum.
由于血清中存在大量高丰度蛋白质，内源性血清肽的选择性提取一直是一项挑战。在此，我们开发了一种简单的在线提取方法，使用强阳离子交换二醇硅胶受限进样材料（SCX-RAM）从人血清中提取肽，并结合二维反相-反相液相色谱质谱联用技术。该在线提取系统操作简便，无需复杂设备。二维反相-反相被证明具有正交性且能高效分离从人血清中提取的肽。
