Wang Shuanglong, Shan Shan, Yang Jian, Zhou Junhui, Wang Guofeng
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, 418 Guanglan Road, Nanchang, 330013, China.
State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants, Changsha, 410019, China.
Anal Bioanal Chem. 2023 Nov;415(27):6701-6709. doi: 10.1007/s00216-023-04947-6. Epub 2023 Sep 27.
A triangular-shaped flat plastic substrate probe was prepared for direct electrospray ionization mass spectrometry (ESI-MS) for analysis of untreated chemical and biological samples including liquids (Met-Arg-Phe-Ala peptide, reserpine, and dodecyl aldehyde), solids (biological samples, traditional Chinese medicine), and powders (roasted coffee, rhizoma coptidis, lotus plumule, and Schisandra sphenanthera). Quantitative analysis of reserpine in water yielded a detection limit of 1 ng mL, dynamic response range within 1-500 ng mL, and linearity of signal response ˃0.9925. Compared to the conventional capillary ESI, this plastic probe ESI offers lower cost of analysis (US $0.0056 per probe), higher sensitivity, lower sample consumption, longer signal duration (>6 min), better reproducibility, signal stability, and higher speed of analysis (<10 s per sample, including sample loading). Overall, the results indicate the potential of ESI-MS based on flat plastic probes as a versatile method for simple, sensitive, and stable analysis of untreated biological sample analysis.
制备了一种三角形扁平塑料基底探针，用于直接电喷雾电离质谱（ESI-MS）分析未处理的化学和生物样品，包括液体（甲硫氨酸-精氨酸-苯丙氨酸-丙氨酸肽、利血平、十二烷基醛）、固体（生物样品、中药）和粉末（烘焙咖啡、黄连、莲子心、五味子）。对水中利血平的定量分析得出检测限为1 ng/mL，动态响应范围在1 - 500 ng/mL之间，信号响应线性度>0.9925。与传统毛细管ESI相比，这种塑料探针ESI提供了更低的分析成本（每个探针0.0056美元）、更高的灵敏度、更低的样品消耗、更长的信号持续时间（>6分钟）、更好的重现性、信号稳定性以及更高的分析速度（每个样品<10秒，包括样品加载）。总体而言，结果表明基于扁平塑料探针的ESI-MS作为一种通用方法，用于未处理生物样品的简单、灵敏和稳定分析具有潜力。
