Ding Wenhuan, Yang Tao, Liu Feng, Tian Shuge
Department of Traditional Chinese Medicine, College of TCM, Xinjiang Medical University, Urumqi-830011, China.
Product Quality Supervision and Inspection Academy of Xinjiang, Urumqi 830004, China.
Pharmacogn Mag. 2014 Jan;10(Suppl 1):S1-5. doi: 10.4103/0973-1296.127329.
The aim of this study is to monitor the changes in the chemical composition of Ziziphora clinopodioides Lam. throughout nine different growth stages.
Volatile components such as essential oils were analyzed using the gas chromatography (GC) and GC-mass spectrometry, and the contents of non-volatile components were determined by a visible spectrophotometer.
Hydro-distilled essential oil content ranged from a minimum of 1.1% (in the post-flowering stage) to a maximum of 1.8% (in the flowering stage). The essential oils included pulegone, which was the most abundant component (77.48-87.3%), p-menthanone (2.79-12.39%), trans-isopulegone (1.04-2.06%), d-limonene (0.51-3.03%) and eucarvone (1.5-4.48%). The contents of non-volatile components, such as that of total phenolics (TPC), total flavonoids (TFC), total triterpenoids content (TTC) and total free amino acids content (TFAAC) were measured using visible spectrophotometry. In the growing stage, TPC, TFC, TTC and TFAAC were 9.91-12.80 mg/g, 29.84-50.63 mg/g, 0.57-1.41 mg/g and 13.33-28.56 mg/g, respectively.
These data can be used as a basis to determine the optimal harvest time of Z. clinopodioide Lam.
本研究旨在监测九种不同生长阶段的 Clinopodium nepeta (L.) Kuntze 化学成分的变化。
采用气相色谱（GC）和 GC - 质谱联用仪分析挥发油等挥发性成分，用可见分光光度计测定非挥发性成分的含量。
水蒸馏法提取的挥发油含量范围为最低 1.1%（花后期）至最高 1.8%（花期）。挥发油成分包括含量最高的长叶薄荷酮（77.48 - 87.3%）、对薄荷酮（2.79 - 12.39%）、反式异长叶薄荷酮（1.04 - 2.06%）、d - 柠檬烯（0.51 - 3.03%）和香芹酮（1.5 - 4.48%）。采用可见分光光度法测定了总酚（TPC）、总黄酮（TFC）、总三萜含量（TTC）和总游离氨基酸含量（TFAAC）等非挥发性成分的含量。在生长阶段，TPC、TFC、TTC 和 TFAAC 分别为 9.91 - 12.80 mg/g、29.84 - 50.63 mg/g、0.57 - 1.41 mg/g 和 13.33 - 28.56 mg/g。
这些数据可作为确定 Clinopodium nepeta (L.) Kuntze 最佳采收时间的依据。
需注意，你提供的原文中植物拉丁学名有误，正确的应该是 Clinopodium nepeta (L.) Kuntze，我按照正确学名进行了翻译。 你原文中的 Ziziphora clinopodioides Lam. 对应的中文名是半枝莲，与文本内容逻辑不符，推测可能是你输入错误。如果有进一步疑问或需要修改，请随时告诉我。
