Biswas Ashok, Dey Susmita, Xiao Aiping, Huang Siqi, Birhanie Ziggiju Mesenbet, Deng Yong, Liu Liangliang, Li Defang
Annual Bast Fiber Breeding Lab., Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China.
Department of Horticulture, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Heliyon. 2023 May 20;9(6):e16494. doi: 10.1016/j.heliyon.2023.e16494. eCollection 2023 Jun.
Different parts of and possess different antioxidant compounds. This study investigated the phytochemical components and antioxidant capacities of ultrasound-assisted extraction of different plant parts of both species using spectrophotometry at various phenological stages. Results also indicate that leaves, stems and roots of at various growth stages showed higher phytochemical components and antioxidant potential compared to . The phytochemical components from roots to leaves in including total polyphenol 0.97-11.11 mg GAE/g DW, total flavonoid 0.99-7.78 mg QE/g DW and total tannin 4.02-26.89 TA E/g DW, whereas total polyphenol 1.04-7.93 mg GAE/g DW, total flavonoid 0.77-5.5.92 mg QE/g DW and total tannin content 3.17-22.73 TA E/g DW. produced overall 22.23%, 13.61%, 12.24% higher total polyphenol, total flavonoid and total tannin, respectively compare to Different parts extract also significantly affected antioxidant capacities including DPPH, ABTS, and FRAP activity with values of 22.03-79.46% inhibition, 10.84-104.10 μmol TE/g DW, and 10.84-104.10 μmol Feg DW respectively for while demonstrated 14.03-70.97% of DPPH inhibition, 9.16-95.60 μmol TE/g DW of ABTS and 5.31-71.82 μmol Feg DW of FRAP activity. Moreover, leaves of the flowering stage, young stems and aged roots of both species displayed a higher content of phytochemical and antioxidant activities than other growth stages. A positive correlation between the phytochemical and antioxidant potential indicated that phenolic constituents solely affected antioxidant activity. Thus, this study established that the plant's parts and phenological growth stages significantly influence the concentration of phytoconstituents and antioxidant activities, and determine the harvesting stages of the different organs of and for considerable medicinal importance as folk and industry.
[物种名称1]和[物种名称2]的不同部位含有不同的抗氧化化合物。本研究利用分光光度法，对这两个物种不同植物部位在不同物候期进行超声辅助提取，研究其植物化学成分和抗氧化能力。结果还表明，[物种名称1]在不同生长阶段的叶、茎和根相比[物种名称2]显示出更高的植物化学成分和抗氧化潜力。[物种名称1]从根到叶的植物化学成分包括总多酚0.97 - 11.11毫克没食子酸当量/克干重、总黄酮0.99 - 7.78毫克槲皮素当量/克干重和总单宁4.02 - 26.89单宁酸当量/克干重，而[物种名称2]的总多酚为1.04 - 7.93毫克没食子酸当量/克干重、总黄酮0.77 - 5.592毫克槲皮素当量/克干重和总单宁含量3.17 - 22.73单宁酸当量/克干重。与[物种名称2]相比，[物种名称1]的总多酚、总黄酮和总单宁分别总体高出22.23%、13.61%、12.24%。不同部位提取物对抗氧化能力也有显著影响，包括DPPH、ABTS和FRAP活性，[物种名称1]的值分别为22.03 - 79.46%抑制率、10.84 - 104.10微摩尔TE/克干重和10.84 - 104.10微摩尔Fe2+/克干重，而[物种名称2]的DPPH抑制率为14.03 - 70.97%、ABTS为9.16 - 95.60微摩尔TE/克干重、FRAP活性为5.31 - 71.82微摩尔Fe2+/克干重。此外，两个物种开花期的叶、幼茎和老根的植物化学成分含量和抗氧化活性均高于其他生长阶段。植物化学成分与抗氧化潜力之间呈正相关，表明酚类成分单独影响抗氧化活性。因此，本研究确定，植物部位和物候生长阶段显著影响植物成分的浓度和抗氧化活性，并确定了[物种名称1]和[物种名称2]不同器官的收获阶段，这在民间和工业医药方面具有相当重要的意义。
