Minonzio F, Ongari A M, Venegoni E, Carbonelli V, Licciardello L, Capsoni F
Istituto di Clinica Medica I, University of Milan.
Boll Ist Sieroter Milan. 1989;68(3):241-8.
D53 (Immucytal) is a compositive vaccine made of immunogenic ribosomes extracted from 4 bacterial species (Klebsiella pneumoniae, Haemophilus influenzae, Streptococcus pyogenes and Streptococcus pneumoniae) associated with a membrane proteoglycan from a non-encapsulated strain of Klebsiella pneumoniae (MPG-Kp). In this work we have studied the effect of the compound on human polymorphonuclear leukocyte (PMN) function "in vitro". We have demonstrated that D53 was able to significantly increase Fc- receptor dependent phagocytosis without modify the C3-receptor dependent activity. Furthermore D53 enhanced the oxidative metabolism (evaluated by chemiluminescence) both using cells in resting conditions or after stimulation with phagocytable or soluble stimuli. On the contrary D53 caused a dose-dependent inhibition of PMN migration toward different chemoattractants. Using the two constitutive fractions of the compound (ribosomes and proteoglycans) we have observed that the MPG-Kp component was mainly responsible for the modulating activity of the drug on human PMNs.
D53（免疫细胞素）是一种复合疫苗，由从4种细菌（肺炎克雷伯菌、流感嗜血杆菌、化脓性链球菌和肺炎链球菌）中提取的免疫原性核糖体与来自肺炎克雷伯菌非包膜菌株的膜蛋白聚糖（MPG-Kp）组成。在这项研究中，我们研究了该化合物对人多形核白细胞（PMN）“体外”功能的影响。我们证明，D53能够显著增加Fc受体依赖性吞噬作用，而不改变C3受体依赖性活性。此外，无论是使用处于静息状态的细胞，还是在用可吞噬或可溶性刺激物刺激后的细胞，D53都能增强氧化代谢（通过化学发光评估）。相反，D53对PMN向不同趋化因子的迁移产生剂量依赖性抑制作用。使用该化合物的两个组成部分（核糖体和蛋白聚糖），我们观察到MPG-Kp成分主要负责该药物对人PMN的调节活性。
