Ballardini G, Fallani M, Bianchi F B, Pisi E
Istituto di Clinica Medica Generale e Terapia Medica, University of Bologna, Italy.
Autoimmunity. 1989;3(2):135-44. doi: 10.3109/08916938909019962.
Although Langerhans, interdigitating and follicular dendritic cells have been occasionally identified in pathological human liver by ultrastructural morphology, no data are available on their phenotypical their phenotypical identification using monoclonal antibodies. Frozen normal liver samples and biopsies from patients with primary biliary cirrhosis, primary sclerosing cholangitis and chronic active hepatitis were studied using a panel of 12 monoclonal antibodies (including anti class II antigens, follicular dendritic, macrophage and Langerhans cells). In normal liver, class II positive cells were represented by Kupffer and portal tract histiocytes with a macrophage phenotype. In pathological portal tracts non lymphocytic class II positive cells were represented by macrophages, clusters of follicular dendritic cells (which were detected in close association with B cell aggregates), and by sparse Langerhans cells (localized in areas of piecemeal necrosis and in a periductal position or infiltrating class II positive bile ducts). The present data suggest that both classical antigen presenting cells and class II positive bile duct cells may play some role in the induction of autoimmune reactions.
尽管通过超微结构形态学偶尔能在病理性人类肝脏中识别出朗格汉斯细胞、交错突细胞和滤泡树突状细胞，但关于使用单克隆抗体对其进行表型鉴定的数据尚无报道。我们使用一组12种单克隆抗体（包括抗II类抗原、滤泡树突状细胞、巨噬细胞和朗格汉斯细胞的抗体）对正常肝脏冷冻样本以及原发性胆汁性肝硬化、原发性硬化性胆管炎和慢性活动性肝炎患者的活检组织进行了研究。在正常肝脏中，II类阳性细胞由具有巨噬细胞表型的库普弗细胞和门管区组织细胞代表。在病理性门管区，非淋巴细胞性II类阳性细胞由巨噬细胞、滤泡树突状细胞簇（在与B细胞聚集物紧密相关处检测到）以及稀疏的朗格汉斯细胞（定位于桥接坏死区域、导管周围位置或浸润II类阳性胆管）代表。目前的数据表明，经典的抗原呈递细胞和II类阳性胆管细胞可能在自身免疫反应的诱导中发挥一定作用。
