Zurgil N, Konikoff F, Bakimer R, Slor H, Shoenfeld Y
Department of Human Genetics, Sackler School of Medicine, Tel Aviv University and BioHytech, Israel.
Autoimmunity. 1989;4(4):289-97. doi: 10.3109/08916938909014705.
Mitochondrial antigens were purified from rat liver and characterized by immunoblotting. Sera from 19 well defined patients with primary biliary cirrhosis (PBC) reacted with two mitochondrial polypeptides of 68 Kd and 45 Kd. Antibodies to these antigens were not detected in any of the sera of patients with cirrhosis of the liver, chronic active hepatitis or other autoimmune diseases. The two polypeptides were derived from the soluble fraction of the mitochondrial matrix. An enzyme-linked immunosorbent assay (ELISA) employing these rat liver mitochondrial antigens is described. Positive results were obtained with all except one PBC sera (95%), five out of 47 patients with cirrhosis (11%), one out of 20 patients with chronic active hepatitis (5%), and two out of 19 patients with various autoimmune disorders (11%). The titers detected in PBC were markedly higher than those recorded in patients with other liver and autoimmune diseases. Strong correlation was found between immunoblotting and the ELISA in determining antimitochondrial antibodies. The ELISA presented is easily performed and seems to be a useful diagnostic tool for antimitochondrial antibodies in patients with PBC.
从大鼠肝脏中纯化线粒体抗原，并通过免疫印迹法对其进行表征。19例明确诊断为原发性胆汁性肝硬化（PBC）的患者血清与两种分子量分别为68 Kd和45 Kd的线粒体多肽发生反应。在肝硬化、慢性活动性肝炎或其他自身免疫性疾病患者的血清中均未检测到针对这些抗原的抗体。这两种多肽来自线粒体基质的可溶部分。本文描述了一种使用这些大鼠肝脏线粒体抗原的酶联免疫吸附测定（ELISA）方法。除1例PBC血清外，所有PBC血清均呈阳性结果（95%）；47例肝硬化患者中有5例（11%）呈阳性；20例慢性活动性肝炎患者中有1例（5%）呈阳性；19例各种自身免疫性疾病患者中有2例（11%）呈阳性。PBC患者检测到的滴度明显高于其他肝脏疾病和自身免疫性疾病患者。在检测抗线粒体抗体方面，免疫印迹法与ELISA之间存在很强的相关性。所介绍的ELISA方法操作简便，似乎是诊断PBC患者抗线粒体抗体的一种有用的诊断工具。
