Gilburd B, Ziporen L, Zharhary D, Blank M, Zurgil N, Scheinberg M A, Guedes L H, Gershwin M E, Shoenfeld Y
Department of Medicine B, Sheba Medical Center, Tel Hashomer, Israel.
J Clin Immunol. 1994 Jan;14(1):14-9. doi: 10.1007/BF01541171.
Sera from 69 patients with leprosy but without liver involvement were assayed for the presence of mitochondrial pyruvate dehydrogenase (PDH)-specific autoantibodies by enzyme-linked immunoabsorbent assay (ELISA), immunoblotting using PDH as an antigen and by enzymatic inhibition test. Twenty-seven of the leprosy serum samples (39.1%) were found to react with PDH by ELISA. However, unlike sera from primary biliary cirrhosis (PBC) patients, none of these were able to inhibit the PDH enzymatic activity. By immunoblotting, it was found that only 2 of the 27 positive sera recognized the 74-kD protein of the PDH complex, which is recognized by sera of most PBC patients. The antimitochondrial antibodies in lepra most probably recognize different epitopes than those in PBC. These findings may indicate that anti-PDH autoantibodies in patients with leprosy may arise by polyclonal B cell stimulation and may represent natural anti-PDH autoantibodies.
采用酶联免疫吸附测定法（ELISA）、以丙酮酸脱氢酶（PDH）为抗原的免疫印迹法以及酶抑制试验，对69例无肝脏受累的麻风病患者的血清进行检测，以确定是否存在线粒体丙酮酸脱氢酶特异性自身抗体。通过ELISA发现，27份麻风病血清样本（39.1%）与PDH发生反应。然而，与原发性胆汁性肝硬化（PBC）患者的血清不同，这些样本均不能抑制PDH的酶活性。通过免疫印迹法发现，27份阳性血清中只有2份识别PDH复合物的74-kD蛋白，而大多数PBC患者的血清能识别该蛋白。麻风病患者的抗线粒体抗体识别的表位很可能与PBC患者的不同。这些发现可能表明，麻风病患者体内的抗PDH自身抗体可能是由多克隆B细胞刺激产生的，可能代表天然的抗PDH自身抗体。
