Arabski Michał, Konieczna Iwona, Tusińska Ewa, Wąsik Sławomir, Relich Inga, Zając Krzysztof, Kamiński Zbigniew J, Kaca Wiesław
Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
Microbiol Res. 2015 Jan;170:242-7. doi: 10.1016/j.micres.2014.05.004. Epub 2014 May 21.
Lysozyme (1,4-β-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur.
