Moore L J, Coleman A W
Division of Biology and Medicine, Brown University, Providence, RI 02912.
Plant Mol Biol. 1989 Oct;13(4):459-65. doi: 10.1007/BF00015557.
A physical restriction map of the mitochondrial genome from one clone (TCC 854) of the sexually isolated populations (syngens) of the morphologically uniform species Pandorina morum Bory has been constructed using restriction endonucleases Ava I, Bam HI, Bgl II, Eco RI, Kpn I, and Pst I. The 20 kb linear genome can easily be separated from plastid DNA, nuclear satellite rDNA, and main band (nuclear) DNA on a Hoechst/CsCl buoyant density gradient. The Pandorina mitochondrial DNA shows sufficient similarity to the 16 kb mitochondrial genome of Chlamydomonas reinhardtii to cross-hybridize, and also hybridizes with a probe containing maize mitochondrial 18S rRNA genes. Double digests, self-probing, and Bal31 exonuclease experiments suggest that 1.8 to 3.3 kb of sequence is repeated at each end of the genome as an inverted repeat. Mitochondrial genome sizes of other P. morum syngens were found to range from ca. 20 to ca. 38 kb. The mitochondrial genome should be valuable for taxonomic studies; it can be used for comparative organellar studies; and it should be of interest to compare with that of other plant and animal mitochondrial genomes.
利用限制性内切酶Ava I、Bam HI、Bgl II、Eco RI、Kpn I和Pst I构建了形态学上一致的物种多细胞实球藻（Pandorina morum Bory）有性隔离种群（同宗配合群）中一个克隆（TCC 854）的线粒体基因组物理限制图谱。在Hoechst/CsCl浮力密度梯度上，20 kb的线性基因组很容易与质体DNA、核卫星rDNA和主带（核）DNA分离。多细胞实球藻线粒体DNA与莱茵衣藻（Chlamydomonas reinhardtii）16 kb的线粒体基因组具有足够的相似性，可以进行交叉杂交，并且还能与含有玉米线粒体18S rRNA基因的探针杂交。双酶切、自身探针杂交和Bal31核酸外切酶实验表明，基因组两端各有1.8至3.3 kb的序列以反向重复的形式存在。发现多细胞实球藻其他同宗配合群的线粒体基因组大小在约20至约38 kb之间。线粒体基因组对于分类学研究应该是有价值的；它可用于比较细胞器研究；并且与其他动植物线粒体基因组进行比较应该会很有趣。
