来自单一动物的牛线粒体DNA物理图谱。
A physical map of bovine mitochondrial DNA from a single animal.
作者信息
Laipis P J, Hauswirth W W, O'Brien T W, Michaels G S
出版信息
Biochim Biophys Acta. 1979 Nov 22;565(1):22-32. doi: 10.1016/0005-2787(79)90080-7.
Mitochondrial DNA has been isolated from the liver of an individual Holstein cow and a physical map has been derived for the 38 cleavage sites made by restriction endonucleases: Ava I, Bam HI, Bgl II, Bst EII, Eco RI, Hha I, Hin dIII, Hpa I, Kpn I, Pst I, Sac I, Sal I, Xba I, and Xho I. Sufficient mitochondrial DNA (approx. 16 mg) could be isolated, allowing this map to serve as the basis for detailed physical, genetic and nucleotide sequence studies in an individual mammal.
已从一头荷斯坦奶牛的肝脏中分离出线粒体DNA，并绘制了由限制性内切酶Ava I、Bam HI、Bgl II、Bst EII、Eco RI、Hha I、Hin dIII、Hpa I、Kpn I、Pst I、Sac I、Sal I、Xba I和Xho I产生的38个切割位点的物理图谱。能够分离出足够的线粒体DNA（约16毫克），使得该图谱可作为对单个哺乳动物进行详细物理、遗传和核苷酸序列研究的基础。
Zotero 插件