A physical map of bovine mitochondrial DNA from a single animal.

Mitochondrial DNA has been isolated from the liver of an individual Holstein cow and a physical map has been derived for the 38 cleavage sites made by restriction endonucleases: Ava I, Bam HI, Bgl II, Bst EII, Eco RI, Hha I, Hin dIII, Hpa I, Kpn I, Pst I, Sac I, Sal I, Xba I, and Xho I. Sufficient mitochondrial DNA (approx. 16 mg) could be isolated, allowing this map to serve as the basis for detailed physical, genetic and nucleotide sequence studies in an individual mammal.