Wesolowski M, Fukuhara H
Institut Curie, Centre Universitaire, Orsay, France.
Mol Cell Biol. 1981 May;1(5):387-93. doi: 10.1128/mcb.1.5.387-393.1981.
The mitochondrial deoxyribonucleic acid (mtDNA) from a petite-negative yeast, Hansenula mrakii, was studied. A linear restriction map was constructed with 11 restriction enzymes. The linearity of the genome was confirmed by direct end labeling of the molecule, followed by restriction analysis. The molecular weight of the DNA was found to be 55,000 base pairs. This is the first linear mtDNA found in yeast species. Using specific gene probes obtained from Saccharomyces cerevisiae mtDNA, we have constructed a gene map of H. mrakii mtDNA. The arrangement of genes in this linear genome was very different from the circular mtDNA of other known yeasts.
对来自小菌落阴性酵母马克斯克鲁维酵母的线粒体脱氧核糖核酸（mtDNA）进行了研究。用11种限制酶构建了线性限制图谱。通过对分子进行直接末端标记，随后进行限制分析，证实了基因组的线性。发现该DNA的分子量为55,000个碱基对。这是在酵母物种中首次发现的线性mtDNA。利用从酿酒酵母mtDNA获得的特异性基因探针，我们构建了马克斯克鲁维酵母mtDNA的基因图谱。该线性基因组中的基因排列与其他已知酵母的环状mtDNA有很大不同。
