Nusa Roy, Prasetyowati Heni, Meutiawati Febrina, Yohan Benediktus, Trimarsanto Hidayat, Setianingsih Tri Yuli, Sasmono R Tedjo
Vector Borne Disease Control, Research and Development Council, Ministry of Health of the Republic of Indonesia, Ciamis, West Java, Indonesia.
J Infect Dev Ctries. 2014 Jun 11;8(6):733-41. doi: 10.3855/jidc.3959.
Dengue is endemic and affects people in all Indonesian provinces. Increasing dengue cases have been observed every year in Sukabumi in West Java province. Despite the endemicity, limited data is available on the genetic of dengue viruses (DENV) circulating in the country. To understand the dynamics of dengue disease, we performed molecular and serological surveillance of dengue in Sukabumi.
A total of 113 patients were recruited for this study. Serological data were obtained using anti-dengue IgM and IgG tests plus dengue NS1 antigen detection. Dengue detection and serotyping were performed using real-time RT-PCR. Viruses were isolated and the envelope genes were sequenced. Phylogenetic and evolutionary analyses were performed to determine the genotype of the viruses and their evolutionary rates.
Real-time RT-PCR detected DENV in 25 (22%) of 113 samples. Serotyping revealed the predominance of DENV-2 (16 isolates, 64%), followed by DENV-1 (5 isolates, 20%), and DENV-4 (4 isolates, 16%). No DENV-3 was detected in the samples. Co-circulation of genotype I and IV of DENV-1 was observed. The DENV-2 isolates all belonged to the Cosmopolitan genotype, while DENV-4 isolates were grouped into genotype II. Overall, their evolutionary rates were similar to DENV from other countries.
We revealed the distribution of DENV serotypes and genotypes in Sukabumi. Compared to data obtained from other cities in Indonesia, we observed the differing predominance of DENV serotypes but similar genotype distribution, where the infecting viruses were closely related with Indonesian endemic viruses isolated previously.
登革热在印度尼西亚呈地方流行性，影响该国所有省份的人群。西爪哇省苏加武眉市每年的登革热病例都在增加。尽管该病呈地方流行性，但关于该国流行的登革热病毒（DENV）基因的可用数据有限。为了解登革热疾病的动态，我们在苏加武眉市开展了登革热的分子和血清学监测。
本研究共招募了113名患者。血清学数据通过抗登革热IgM和IgG检测以及登革热NS1抗原检测获得。使用实时逆转录聚合酶链反应（RT-PCR）进行登革热检测和血清分型。分离病毒并对包膜基因进行测序。进行系统发育和进化分析以确定病毒的基因型及其进化速率。
实时RT-PCR在113份样本中的25份（22%）中检测到DENV。血清分型显示DENV-2占主导（16株，64%），其次是DENV-1（5株，20%）和DENV-4（4株，16%）。样本中未检测到DENV-3。观察到DENV-1的基因型I和IV共同流行。DENV-2分离株均属于泛在基因型，而DENV-4分离株被归为基因型II。总体而言，它们的进化速率与其他国家的DENV相似。
我们揭示了苏加武眉市DENV血清型和基因型的分布。与从印度尼西亚其他城市获得的数据相比，我们观察到DENV血清型的优势不同，但基因型分布相似，其中感染病毒与先前分离的印度尼西亚地方流行病毒密切相关。
