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从脑炎患者的脑脊液中分离出神经嗜性登革热病毒血清型 3 并进行全基因组分析。

Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient.

机构信息

Eijkman Institute for Molecular Biology, Jakarta, Indonesia.

Department of Paediatrics, Dr. Cipto Mangunkusumo National Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

出版信息

PLoS Negl Trop Dis. 2018 Jan 12;12(1):e0006198. doi: 10.1371/journal.pntd.0006198. eCollection 2018 Jan.

DOI:10.1371/journal.pntd.0006198
PMID:29329287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5809095/
Abstract

Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus.

摘要

虽然已经报道了与登革热病毒（DENV）感染相关的神经表现，但关于亲神经 DENV 的遗传特征的信息非常有限。在这里，我们描述了从印度尼西亚雅加达一名脑炎儿科患者的脑脊液中分离和完全基因组分析的血清型 3（DENV-3）。我们使用下一代测序来推断亲神经 DENV-3 分离株的完整基因组。基于完整基因组分析，在病毒的蛋白编码区观察到两个独特的和九个不常见的氨基酸变化。系统发育树和分子钟分析表明，该亲神经病毒是 DENV-3 基因型 I 的苏门答腊-爪哇枝的成员，与 1998 年左右来自雅加达的其他分离株有共同的祖先。这是首次对亲神经 DENV-3 完整基因组分析的报道，提供了有关该病毒遗传特征的详细信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/ccd223ab1cf0/pntd.0006198.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/8324649fe819/pntd.0006198.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/c4299e7df585/pntd.0006198.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/ccd223ab1cf0/pntd.0006198.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/8324649fe819/pntd.0006198.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/c4299e7df585/pntd.0006198.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/893f/5809095/ccd223ab1cf0/pntd.0006198.g003.jpg

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1
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2
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Pathog Glob Health. 2016 May;110(3):119-29. doi: 10.1080/20477724.2016.1184864. Epub 2016 May 23.
3
Meta-Analysis of Dengue Severity during Infection by Different Dengue Virus Serotypes in Primary and Secondary Infections.
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BMC Infect Dis. 2024 Mar 15;24(1):319. doi: 10.1186/s12879-024-09198-z.
4
Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route.经鼻腔途径在实验幼鼠和成年鼠体内有效感染登革病毒。
Viruses. 2022 Jun 26;14(7):1394. doi: 10.3390/v14071394.
5
Detection of dengue virus serotype 1 in central nervous system of a child in Bandung, West Java: A case report.西爪哇省万隆市一名儿童中枢神经系统中登革热病毒1型的检测：病例报告
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6
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7
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5
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8
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9
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