Wardhani Puspa, Aryati Aryati, Yohan Benediktus, Trimarsanto Hidayat, Setianingsih Tri Y, Puspitasari Dwiyanti, Arfijanto Muhammad Vitanata, Bramantono Bramantono, Suharto Suharto, Sasmono R Tedjo
Department of Clinical Pathology, School of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Institute for Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia.
PLoS One. 2017 Jun 2;12(6):e0178443. doi: 10.1371/journal.pone.0178443. eCollection 2017.
Dengue disease is still a major health problem in Indonesia. Surabaya, the second largest city in the country, is endemic for dengue. We report here on dengue disease in Surabaya, investigating the clinical manifestations, the distribution of dengue virus (DENV) serotypes, and the relationships between clinical manifestations and the genetic characteristics of DENV. A total of 148 patients suspected of having dengue were recruited during February-August 2012. One hundred one (68%) of them were children, and 47 (32%) were adults. Dengue fever (DF) and Dengue hemorrhagic fever (DHF) were equally manifested in all of the patients. We performed DENV serotyping on all of the samples using real-time RT-PCR. Of 148, 79 (53%) samples were detected as DENV positive, with DENV-1 as the predominant serotype (73%), followed by DENV-2 (8%), DENV-4 (8%), and DENV-3 (6%), while 5% were mixed infections. Based on the Envelope gene sequences, we performed phylogenetic analyses of 24 isolates to genotype the DENV circulating in Surabaya in 2012, and the analysis revealed that DENV-1 consisted of Genotypes I and IV, DENV-2 was of the Cosmopolitan genotype, the DENV-3 viruses were of Genotype I, and DENV-4 was detected as Genotype II. We correlated the infecting DENV serotypes with clinical manifestations and laboratory parameters; however, no significant correlations were found. Amino acid analysis of Envelope protein did not find any unique mutations related to disease severity.
登革热疾病在印度尼西亚仍然是一个主要的健康问题。该国第二大城市泗水是登革热的地方性流行区。我们在此报告泗水的登革热疾病情况，调查其临床表现、登革热病毒（DENV）血清型分布以及临床表现与DENV基因特征之间的关系。2012年2月至8月期间，共招募了148名疑似登革热患者。其中101名（68%）为儿童，47名（32%）为成人。登革热（DF）和登革出血热（DHF）在所有患者中的表现相同。我们使用实时RT-PCR对所有样本进行了DENV血清分型。在148个样本中，79个（53%）样本被检测为DENV阳性，其中DENV-1为主要血清型（73%），其次是DENV-2（8%）、DENV-4（8%）和DENV-3（6%），5%为混合感染。基于包膜基因序列，我们对24株分离株进行了系统发育分析，以确定2012年在泗水流行的DENV基因型，分析显示DENV-1由基因型I和IV组成，DENV-2为泛基因型，DENV-3病毒为基因型I，DENV-4被检测为基因型II。我们将感染的DENV血清型与临床表现和实验室参数进行了关联；然而，未发现显著相关性。包膜蛋白的氨基酸分析未发现与疾病严重程度相关的任何独特突变。
