Chen Li-Qin, Wang Yu, Qu Jian-Song, Deng Jian-Jun, Kang Xue-Jun
School of Public Health, Tianjin Medical University, Tianjin, 300070, China; Key Laboratory of Child Development and Learning Science (Ministry of Education), Research Center for Learning Science, Southeast University, Nanjing, 210096, China.
Biomed Chromatogr. 2015 Jan;29(1):103-9. doi: 10.1002/bmc.3245. Epub 2014 Jun 11.
For the first time, electrospun composite nanofibers comprising polymeric crown ether with polystyrene (PCE-PS) have been used for the selective extraction of catecholamines - dopamine (DA), norepinephrine (NE) and epinephrine (E) - prior to their analysis by high-performance liquid chromatography-electrochemical detection. Using a minicartridge packed with PCE-PS composite nanofibers, the target compounds were extracted effectively from urine samples to which diphenylborinic acid 2-aminoethyl ester was added as a complexing reagent. The extracted catecholamines could be liberated from the fiber by the addition of acetic acid. A good linearity was observed for catecholamines in the range of 2.0-200 ng mL(-1) (NE, E and DA). The detection limits of catecholamines (signal-to-noise ratio = 3) were 0.5 ng mL(-1) (NE), 0.2 ng mL(-1) (E) and 0.2 ng mL(-1) (DA), respectively. Under the optimized conditions, the absolute recoveries of the above three catecholamines were 90.6% (NE), 88.5% (E) and 94.5% (DA). The repeatability of extraction performance was from 5.4 to 9.2% (expressed as relative standard deviation). Our results indicate that the proposed method could be used for the determination of NE, E and DA in urine.
首次将包含聚合物冠醚与聚苯乙烯（PCE-PS）的电纺复合纳米纤维用于在通过高效液相色谱-电化学检测进行分析之前选择性萃取儿茶酚胺——多巴胺（DA）、去甲肾上腺素（NE）和肾上腺素（E）。使用填充有PCE-PS复合纳米纤维的微型柱，目标化合物从添加了二苯基硼酸2-氨基乙酯作为络合剂的尿液样本中被有效萃取。通过添加乙酸可使萃取的儿茶酚胺从纤维中释放出来。观察到儿茶酚胺在2.0 - 200 ng mL⁻¹范围内具有良好的线性（NE、E和DA）。儿茶酚胺的检测限（信噪比 = 3）分别为0.5 ng mL⁻¹（NE）、0.2 ng mL⁻¹（E）和0.2 ng mL⁻¹（DA）。在优化条件下，上述三种儿茶酚胺的绝对回收率分别为90.6%（NE）、88.5%（E）和94.5%（DA）。萃取性能的重复性为5.4%至9.2%（以相对标准偏差表示）。我们的结果表明，所提出的方法可用于测定尿液中的NE、E和DA。
