Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin Medical University, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China.
Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin Medical University, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Feb 15;1139:121983. doi: 10.1016/j.jchromb.2020.121983. Epub 2020 Jan 18.
Despite the development of an off-line packed fiber solid phase extraction procedure (PFSPE) for urinary catecholamines, automation remains a challenge. Here, we propose an on-line PFSPE-HPLC procedure for automated sample processing and analysis of urinary catecholamines, with good recovery and precision, to avoid manual operation errors. The on-line PFSPE-HPLC procedure has been thoroughly optimized concerning the gradient, valve switch timing, the effects of complexing reagent and buffer solution, and the stability of the nanofibers. Validation of the developed on-line PFSPE-HPLC protocol in urine yielded satisfactory accuracies of 99.6-104.2%, precision below 7.0%, as well as a linear range from 1 ng/mL to 100 ng/mL with a correlation coefficient of 0.999. The developed protocol is herein presented as a potential technology for automated sample pretreatment for the determination of urinary catecholamines.
尽管已经开发出了一种用于尿儿茶酚胺的离线填充纤维固相萃取程序（PFSPE），但自动化仍然是一个挑战。在这里，我们提出了一种用于自动处理尿液儿茶酚胺样品并进行分析的在线 PFSPE-HPLC 程序，该程序具有良好的回收率和精密度，可避免手动操作误差。已经针对梯度、阀切换时间、络合剂和缓冲溶液的影响以及纳米纤维的稳定性对在线 PFSPE-HPLC 程序进行了彻底优化。在尿液中对开发的在线 PFSPE-HPLC 方案进行的验证表明，其准确度为 99.6-104.2%，精密度低于 7.0%，线性范围为 1ng/mL 至 100ng/mL，相关系数为 0.999。本研究提出的方案为用于自动预处理尿液儿茶酚胺样品的潜在技术。
