Laboratory of Experimental Cancer Research (LECR), Ghent University, De Pintelaan 185 1P7, B-9000, Ghent, Belgium,
Appl Microbiol Biotechnol. 2014 Aug;98(15):6831-46. doi: 10.1007/s00253-014-5841-1. Epub 2014 Jun 11.
Crosstalk between the human host and its microbiota is reported to influence various diseases such as mucositis. Fundamental research in this area is however complicated by the time frame restrictions during which host-microbe interactions can be studied in vitro. The model proposed in this paper, consisting of an oral epithelium and biofilm, can be used to study microbe-host crosstalk in vitro in non-infectious conditions up to 72 h. Microbiota derived from oral swabs were cultured on an agar/mucin layer and challenged with monolayers of keratinocytes grown on plastic or collagen type I layers embedded with fibroblasts. The overall microbial biofilm composition in terms of diversity remained representative for the oral microbiome, whilst the epithelial cell morphology and viability were unaffected. Applying the model to investigate wound healing revealed a reduced healing of 30 % in the presence of microbiota, which was not caused by a reduction of the proliferation index (52.1-61.5) or a significantly increased number of apoptotic (1-1.13) or necrotic (32-30.5 %) cells. Since the model allows the separate study of the microbial and cellular exometabolome, the biofilm and epithelial characteristics after co-culturing, it is applicable for investigations within fundamental research and for the discovery and development of agents that promote wound healing.
人体宿主与其微生物群之间的相互作用被报道会影响多种疾病，如黏膜炎。然而，由于宿主-微生物相互作用在体外研究的时间限制，该领域的基础研究变得复杂。本文提出的模型由口腔上皮组织和生物膜组成，可用于在非感染条件下体外研究长达 72 小时的微生物-宿主串扰。从口腔拭子中分离的微生物群在琼脂/粘蛋白层上培养，并与在塑料或嵌入成纤维细胞的 I 型胶原层上生长的角质形成细胞单层进行挑战。微生物生物膜的总体多样性组成仍然代表了口腔微生物组，而上皮细胞形态和活力不受影响。应用该模型研究伤口愈合时发现，存在微生物时愈合减少了 30%，这不是由于增殖指数（52.1-61.5）降低或凋亡（1-1.13）或坏死（32-30.5%）细胞数量显著增加所致。由于该模型允许对微生物和细胞外代谢组进行单独研究，因此可用于共培养后生物膜和上皮特性的研究，适用于基础研究以及促进伤口愈合的试剂的发现和开发。
