Song Ze He, Xiao Kan, Ke Ya Lu, Jiao Le Fei, Hu Cai Hong
Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China.
Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China
Innate Immun. 2015 May;21(4):341-8. doi: 10.1177/1753425914536450. Epub 2014 Jun 10.
Weaning is the most significant event in the life of pigs and is always related with intestinal disruption. Although it is well known that zinc oxide (ZnO) exerts beneficial effects on the intestinal barrier, the mechanisms underlying these effects have not yet been fully elucidated. We examined whether ZnO protects the intestinal barrier via mitogen-activated protein kinases and TGF-β1 signaling pathways. Twelve barrows weaned at 21 d of age were randomly assigned to two treatments (0 verus 2200 mg Zn/kg from ZnO) for 1 wk. The results showed that supplementation with ZnO increased daily gain and feed intake, and decreased postweaning scour scores. ZnO improved intestinal morphology, as indicated by increased villus height and villus height:crypt depth ratio, and intestinal barrier function, indicated by increased transepithelial electrical resistance and decreased mucosal-to-serosal permeability to 4-ku FITC dextran. ZnO decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38), while it increased the ratio of ERK (p-ERK/ERK). Supplementation with ZnO increased intestinal TGF-β1 expression. The results indicate that supplementation with ZnO activates ERK ½, and inhibits JNK and p38 signaling pathways, and increases intestinal TGF-β1 expression in weaned pigs.
断奶是猪生命中最重要的事件，且总是与肠道功能紊乱相关。尽管众所周知氧化锌（ZnO）对肠道屏障有有益作用，但其作用机制尚未完全阐明。我们研究了ZnO是否通过丝裂原活化蛋白激酶和TGF-β1信号通路保护肠道屏障。将12头21日龄断奶的公猪随机分为两种处理（0与2200 mg Zn/kg的ZnO），为期1周。结果表明，补充ZnO可提高日增重和采食量，并降低断奶后腹泻评分。ZnO改善了肠道形态，表现为绒毛高度增加和绒毛高度与隐窝深度比值增加，以及肠道屏障功能，表现为跨上皮电阻增加和对4-ku FITC葡聚糖的黏膜到浆膜通透性降低。ZnO降低了磷酸化JNK和p38与总JNK和p38的比值（p-JNK/JNK和p-p38/p38），而增加了ERK的比值（p-ERK/ERK）。补充ZnO可增加肠道TGF-β1表达。结果表明，补充ZnO可激活断奶仔猪的ERK ½，抑制JNK和p38信号通路，并增加肠道TGF-β1表达。
