Aston Emily J, Mayor Pedro, Bowman Dwight D, Mohammed Hussni O, Liotta Janice L, Kwok Oliver, Dubey J P
Cornell University College of Veterinary Medicine, S2-009 Schurman Hall, Ithaca, NY 14853, United States ; National Service of Agrarian Health (SENASA), Av. La Molina N° 1915, Lima 12, La Molina, Lima, Peru.
Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Int J Parasitol Parasites Wildl. 2013 Dec 22;3(1):15-9. doi: 10.1016/j.ijppaw.2013.12.001. eCollection 2014 Apr.
Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them.
弓形虫病是一种由原生动物刚地弓形虫引起的人畜共患病，在全球范围内均有发现。为了确定有蹄类动物是否为秘鲁亚马逊东北部一个偏远孤立地区的刚地弓形虫宿主，通过改良凝集试验（MAT）对5种有蹄类动物的弓形虫抗体进行了检测。这些动物是秘鲁亚马逊东北部亚瓦里-米林河沿岸的自给自足型猎人捕杀的对象。猎人在滤纸上采集血样。为了检测弓形虫抗体，将血液从滤纸上洗脱下来，滴度为1:25被认为表明接触过弓形虫。在26只（31.0%）西猯（白唇西猯、草原西猯）、6只（17.1%）南美泽鹿（美洲泽鹿、低地泽鹿）和4只（40.0%）低地貘中发现了弓形虫抗体。我们还对MAT方案进行了改进，使其能够从几种实验室级滤纸上提取液体样本，从而使研究人员能够轻松地根据所获得的材料调整其方法。
