Zhang Shuijun, Cao Shengli, Wang Tao, Yan Bing, Lu Yantao, Zhao Yongfu
From the Department of Hepatobiliary and Pancreatic Surgery and the Key Laboratory of Hepatobiliary and Pancreatic Surgery & Digestive Organ Transplantation of Henan Province, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Exp Clin Transplant. 2014 Oct;12(5):469-73. doi: 10.6002/ect.2013.0229. Epub 2014 Jun 9.
Experimental animal models of brain death that mimic human conditions may be useful for investigating novel strategies that increase quality and quantity of organs for transplant.
Brain death was induced by increasing intracranial pressure by inflating an intracranial placed balloon catheter. Brain death was confirmed by flatline electroencephalogram, physical signs of apnea, and absence of brain stem reflexes. Donor management was done after brain death. Intracranial pressure and physiologic variables were continually monitored during 9 hours' follow-up.
Ninety percent of brain dead animals showed typical signs of brain death such as diabetes insipidus, hypertensive, and hypotensive periods. Donor care was performed for 9 hours after brain death, and the mean arterial pressure was maintained above 60 mm Hg.
We conclude that the rat model of brain death can be performed in a standardized, reproducible, and successful way.
模拟人类情况的脑死亡实验动物模型可能有助于研究增加移植器官质量和数量的新策略。
通过向颅内放置的球囊导管充气来增加颅内压，从而诱导脑死亡。通过脑电图呈直线、呼吸暂停的体征以及脑干反射消失来确认脑死亡。在脑死亡后进行供体管理。在9小时的随访期间持续监测颅内压和生理变量。
90%的脑死亡动物表现出典型的脑死亡体征，如尿崩症、高血压期和低血压期。在脑死亡后进行了9小时的供体护理，平均动脉压维持在60毫米汞柱以上。
我们得出结论，脑死亡大鼠模型可以以标准化、可重复且成功的方式进行。
