Roschger Andreas, Gamsjaeger Sonja, Hofstetter Birgit, Masic Admir, Blouin Stéphane, Messmer Phaedra, Berzlanovich Andrea, Paschalis Eleftherios P, Roschger Paul, Klaushofer Klaus, Fratzl Peter
Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, AustriabMax Planck Institute of Colloids and Interfaces, 14424 Pot.
Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Straße 30, A-1140 Vienna, Austria.
J Biomed Opt. 2014 Jun;19(6):065002. doi: 10.1117/1.JBO.19.6.065002.
Raman microspectroscopy and quantitative backscattered electron imaging (qBEI) of bone are powerful tools to investigate bone material properties. Both methods provide information on the degree of bone matrix mineralization. However, a head-to-head comparison of these outcomes from identical bone areas has not been performed to date. In femoral midshaft cross sections of three women, 99 regions (20×20 μm²) were selected inside osteons and interstitial bone covering a wide range of matrix mineralization. As the focus of this study was only on regions undergoing secondary mineralization, zones exhibiting a distinct gradient in mineral content close to the mineralization front were excluded. The same regions were measured by both methods. We found a linear correlation (R²=0.75) between mineral/matrix as measured by Raman spectroscopy and the wt. %Mineral/(100-wt. %Mineral) as obtained by qBEI, in good agreement with theoretical estimations. The observed deviations of single values from the linear regression line were determined to reflect biological heterogeneities. The data of this study demonstrate the good correspondence between Raman and qBEI outcomes in describing tissue mineralization. The obtained correlation is likely sensitive to changes in bone tissue composition, providing an approach to detect potential deviations from normal bone.
骨的拉曼光谱和定量背散射电子成像（qBEI）是研究骨材料特性的有力工具。这两种方法都能提供有关骨基质矿化程度的信息。然而，迄今为止尚未对相同骨区域的这些结果进行直接比较。在三名女性的股骨干中段横截面中，在骨单位和骨间质内选择了99个区域（20×20μm²），这些区域涵盖了广泛的基质矿化范围。由于本研究仅关注经历二次矿化的区域，因此排除了在矿化前沿附近矿含量呈现明显梯度的区域。两种方法测量的是相同区域。我们发现，拉曼光谱测量的矿物质/基质与qBEI获得的矿物质重量百分比/（100 - 矿物质重量百分比）之间存在线性相关性（R² = 0.75），与理论估计值高度一致。确定单个值与线性回归线的观察偏差反映了生物异质性。本研究数据表明，拉曼光谱和qBEI结果在描述组织矿化方面具有良好的对应关系。所获得的相关性可能对骨组织组成的变化敏感，为检测与正常骨的潜在偏差提供了一种方法。
