van Alphen M M, van de Kant H J, de Rooij D G
Department of Cell Biology, State University of Utrecht, Medical School, The Netherlands.
Cancer Res. 1989 Feb 1;49(3):533-6.
In adult rhesus monkeys a two- to threefold increase in the number of spermatogonia was found at Day 75 after 1 Gy of X-irradiation when the animals were pretreated with two intramuscular injections of follicle-stimulating hormone (FSH) each day. Also the percentage of cross-sections of seminiferous tubules showing spermatogonia (repopulation index) was much higher when FSH was given before irradiation. At 75 days postirradiation the repopulation index was 39 +/- 10% after irradiation alone and 81 +/- 11% when FSH pretreatment was applied. The pretreatment with two injections of FSH each day during 16 days caused an increase in the number of proliferating A spermatogonia. In view of earlier results in the mouse, where proliferating spermatogonial stem cells appeared more radioresistant than quiescent ones, it is suggested that the protective effects of FSH treatment are caused by the increase in the proliferative activity of the A spermatogonia and consequently of the spermatogonial stem cells. The results indicate that in the rhesus monkey the maximal protective effect of FSH is reached after a period of treatment between 7 and 16 days.
在成年恒河猴中，当每天对动物进行两次肌肉注射促卵泡激素（FSH）预处理后，1 Gy X射线照射75天后精原细胞数量增加了两到三倍。而且，在照射前给予FSH时，显示精原细胞的生精小管横截面积百分比（再增殖指数）要高得多。照射后75天，单独照射后的再增殖指数为39±10%，应用FSH预处理时为81±11%。在16天内每天两次注射FSH进行预处理，导致增殖性A型精原细胞数量增加。鉴于在小鼠中早期的研究结果，即增殖性精原干细胞比静止性精原干细胞表现出更强的辐射抗性，有人提出FSH治疗的保护作用是由A型精原细胞以及精原干细胞增殖活性的增加所引起的。结果表明，在恒河猴中，FSH在7至16天的治疗期后达到最大保护效果。
