van Alphen M M, van de Kant H J, de Rooij D G
Department of Cell Biology, State University of Utrecht Medical School, The Netherlands.
Endocrinology. 1988 Sep;123(3):1449-55. doi: 10.1210/endo-123-3-1449.
A 2-fold increase in the numbers of germinal cells was observed in the seminiferous epithelium of cynomolgus monkeys treated with 15 IU FSH twice a day during 28 days. No effect was seen after 7 days of treatment. After 16 days only the numbers of Apale (Ap) spermatogonia had increased to 200% of the control level while the numbers of B spermatogonia, spermatocytes, and spermatids had increased less (160%, 129%, and 100% of the control level, respectively). In the rhesus monkey after the same dose of FSH an increase in the number of Ap spermatogonia to 152% was found after 16 days. When a dose of 25 IU FSH was administered to cynomolgus monkeys three times per week for 16 days the number of Ap spermatogonia increased to only 131% of the control level. After all treatments no effect on the number of Adark (Ad) spermatogonia was found. It was concluded that the increased levels of plasma FSH caused a specific increase in the number of Ap spermatogonia. The increased number of A spermatogonia gave rise to an increase in the number of B spermatogonia after 16 days of treatment which in turn produced more spermatocytes between 16 and 28 days of treatment. If the FSH was administered for a period of 28 days the number of round spermatids also showed a 2-fold increase. These findings indicate a correlation between plasma FSH levels and the numbers of germinal cells in the seminiferous epithelium. In monkeys treated with 450 IU human CG daily no effect on the numbers of the A spermatogonia was observed.
在28天内每天两次给予15国际单位促卵泡激素（FSH）治疗的食蟹猴的生精上皮中，观察到生殖细胞数量增加了两倍。治疗7天后未见效果。16天后，仅淡染A型精原细胞（Ap）数量增加至对照水平的200%，而B型精原细胞、精母细胞和精子细胞数量增加较少（分别为对照水平的160%、129%和100%）。在给予相同剂量FSH的恒河猴中，16天后发现Ap精原细胞数量增加至152%。当每周三次给食蟹猴注射25国际单位FSH，持续16天时，Ap精原细胞数量仅增加至对照水平的131%。所有治疗后，未发现对暗染A型精原细胞（Ad）数量有影响。得出的结论是，血浆FSH水平升高导致Ap精原细胞数量特异性增加。治疗16天后，A型精原细胞数量增加导致B型精原细胞数量增加，进而在治疗的16至28天内产生更多精母细胞。如果给予FSH 28天，圆形精子细胞数量也会增加两倍。这些发现表明血浆FSH水平与生精上皮中生殖细胞数量之间存在相关性。在每天给予450国际单位人绒毛膜促性腺激素（hCG）治疗的猴子中，未观察到对A型精原细胞数量有影响。
